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 Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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 Cactus tissue culture.
#1932583 - 09/19/03 01:32 PM (5 years, 3 months ago) |
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Here's my first success.
San pedro developing new buds in vitro 
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 neuro
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Re: Cactus tissue culture. [Re: Una]
#1932590 - 09/19/03 01:33 PM (5 years, 3 months ago) |
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what's your medium and hormone content and ratio?
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Una
controlleddemolition
Registered: 03/01/01
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Re: Cactus tissue culture. [Re: neuro]
#1932650 - 09/19/03 01:44 PM (5 years, 3 months ago) |
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I can not reveal the recipe but if you are interested in cactus tissue culture this document will give some very usefull tips.
http://plant-tc.coafes.umn.edu/listserv/2000/log0010/msg00077.html
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neuro
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Re: Cactus tissue culture. [Re: Una]
#1932680 - 09/19/03 01:50 PM (5 years, 3 months ago) |
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heh i already know how to do cultures and such... back in school i had this shitty lab every tuesday afternoon and all we did was make cultures.. i was just curious as to your recipe...but if mum's the word, then mum's the word 
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DazedSol
old hand
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Re: Cactus tissue culture. [Re: Una]
#1933189 - 09/19/03 04:47 PM (5 years, 3 months ago) |
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Very fucken cool Una!!!
Please keep us updated on your progress.......
This is on my (really long)list of things to do(different, harder to acquire cacti though)......
anywho.
Hehe, I was gonna ask the same thing as neuro but figured the request would be shot down  With good reason though,..there is a lot of money to made on successful culture formula's ....plus all the time done experimenting and all.........thats alot to just give away
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theshiftingwalls
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Re: Cactus tissue culture. [Re: DazedSol]
#1933403 - 09/19/03 06:12 PM (5 years, 3 months ago) |
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Give us the secrect... Or thats just fucked...
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theshiftingwalls
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Malt extract or agar?
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neuro
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LOL! Not quite!
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felixhigh
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Re: Cactus tissue culture. [Re: neuro]
#1933797 - 09/19/03 09:41 PM (5 years, 3 months ago) |
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hehehehe
yeah, MEA, then you insert the Cube spores into the mix and there you have a shroomocactus psychodelicus. =)
very very cool UNA!
could you guys please give us more good links on cactus tissue culture? should i treat them like any other plant then (regarding substrate)?
Edited by felixhigh (09/20/03 01:28 AM)
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: felixhigh]
#1935653 - 09/20/03 02:21 PM (5 years, 3 months ago) |
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Of course the medium is not MEA.... That's for fungal growth not for plant growth (although malt extraxt is sometimes added to tissue culture media as an 'undefined' growth factor).
The cactus medium is based on Murashige and Skoog (MS) salts, sugar (sucrose), agar and growth regulators.
I've searched for many hours on the internet for info on TC of cactus but there's not much info out there. The link i posted earlier sums up (practically) all the info that is out there.
Whenever i have the time i will go visit the library of the Agricultural University in Wageningen because i'm sure that many scientific articles have been published about the subject that i can get my hands on there.
As for me not wanting to share the exaxt recipe; This is something we might want to take to a commerical scale (sometimes). In fact i studied horticulture for 4 years and i did my final thesis on the development of plant tissue culture protocols
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neuro
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Re: Cactus tissue culture. [Re: Una]
#1935877 - 09/20/03 04:03 PM (5 years, 3 months ago) |
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>>As for me not wanting to share the exaxt recipe; This is something we might want to take to a commerical scale (sometimes). In fact i studied horticulture for 4 years and i did my final thesis on the development of plant tissue culture protocols
Ahh so this is a carefully investigated recipe.. i understand now..
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theshiftingwalls
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Re: Cactus tissue culture. [Re: neuro]
#1936033 - 09/20/03 05:04 PM (5 years, 3 months ago) |
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Told you he used agar and malt extract.
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Una
controlleddemolition
Registered: 03/01/01
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Quote:
Told you he used agar and malt extract. Quote:
I use malt extract for the growth of fungi, haven't used it for TC plants yet. Malt extract is regarded as complex organic supplement of which the effects are not completely understood.
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Una
controlleddemolition
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Re: Cactus tissue culture. [Re: Una]
#1938225 - 09/21/03 03:29 PM (5 years, 3 months ago) |
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Here's some more pics.....
more pachanoi
Lophophora developing a new pup
Pereskiopsis rooting stage
oh here's some Salvia divinorum (not a cactus i know)
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neuro
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Re: Cactus tissue culture. [Re: Una]
#1938533 - 09/21/03 06:17 PM (5 years, 3 months ago) |
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looks good una..
have you tried producing a callus then growing shoots or roots off the callus in a separate culture?
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ISH
PsilocybianTranslationServices
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Re: Cactus tissue culture. [Re: neuro]
#1938571 - 09/21/03 06:33 PM (5 years, 3 months ago) |
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Bad Ass, I'm very interested in seeing your progress.
ISH
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Agarico
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 Re: Cactus tissue culture. [Re: Una]
#1938577 - 09/21/03 06:36 PM (5 years, 3 months ago) |
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Is it possible to do this without aureoles? Just tissue? 
Cloning Salvia that way looks like everything else than a productive method would 
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Effed
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Re: Cactus tissue culture. [Re: Una]
#1939019 - 09/21/03 09:34 PM (5 years, 3 months ago) |
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Una, you are a true innovator. 
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psilocybe cubensis
Blood, guts, andbroken teeth.
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Re: Cactus tissue culture. *DELETED* [Re: Effed]
#1939068 - 09/21/03 09:58 PM (5 years, 3 months ago) |
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Post deleted by psilocybe cubensis
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Openminded
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Very, very nice. I've always been fascinated by this sort of tissue culture. I love the way you can simply slice off a bit of something and it will grow into a whole new plant. Well, not quite that simple in practice...
I love growing things, but feel more at home in the lab than in the garden, so this would be a perfect hobby for me!
Please, do keep us informed about your progress. How long did it take for the new shoots to form?
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Anand
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Re: Cactus tissue culture. [Re: Openminded]
#1943811 - 09/23/03 09:30 AM (5 years, 3 months ago) |
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Una: will MushMush sell plant Tissue Culture supply's in the future? would be nice.... don't know where to get the MS and other ingredients
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theshiftingwalls
Divine state
Registered: 06/18/03
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Re: Cactus tissue culture. [Re: Anand]
#1946529 - 09/23/03 11:44 PM (5 years, 3 months ago) |
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He would sell the ingredients to make it possible to culture, get it?
Its all marketing  .
Una you better ship to Canada ! You better!
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Embryogenesis from callus is possible with many species so i don't see why it's not possible with cacti. Of course you would need a good protocol (which i don't have yet).
The shoots of the SP took 3 weeks to develop.
We will not be selling plant TC supplies since most of the growth regulators that are needed are toxic (carry the skull icon) and these would require special (expensive) transport.
Anand if you are really serious about this is might help you out with some stuff. Or you might contact :info@labassociates.com at http://www.labassociates.com/
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Una
controlleddemolition
Registered: 03/01/01
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Re: Cactus tissue culture. [Re: Una]
#1957498 - 09/27/03 03:05 PM (5 years, 3 months ago) |
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I set up some pieces yesterday of this cristate/monstrosus pachanoi.
Here's my flow cabinet. Jars wiith medium and sterilised water on the right. Glass bead heater and more sterilised water on the right:
Surface sterilisation with bleach (toilet cleaner )
Rinsing with sterile water.
Notice the bleach damage (white tissue)
The damaged tissue is cut off. And the remaining piece is divided.
These pieces are put on the multiplication/initiation medium.
No i cross my fingers and hope some of the areoles develop new buds.
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Anonymous
Unregistered
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Re: Cactus tissue culture. [Re: Una]
#1958488 - 09/27/03 10:31 PM (5 years, 3 months ago) |
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Very interesting stuff. I was talking to my grandmother the other day about growing mushrooms on agar. Then she starts talking about how my greatgrandfather when he was youger got into orchids. He had a full lab and spent all this money on growing thousands of rare orchids. No hybrids. Just pure strains. Then when Hawai came into the picture the market got flooded with orchids. So since he considered himself an artist he took all his plants and equipment and threw them in a pile and burnet them. I wish I had that stuff. Crazy
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: Una]
#1990544 - 10/08/03 03:50 PM (5 years, 3 months ago) |
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Here's an update on the San Pedro experiment.
I transferred the pieces to fresh medium and cut away some of the callus. The new shoots are developing steadily:
The Trichocereus bridgesii is now also showing some activity:
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Anonymous
Unregistered
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Re: Cactus tissue culture. [Re: neuro]
#2027654 - 10/21/03 01:33 AM (5 years, 2 months ago) |
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Callus yields genetic variation. Bad idea for maintianing cultivars. Good idea for discovering new ones though.
Stick with the axillary shoot multiplication for maintaining cultivars, while multiplying them.
Those look nice Una.
Research is very limited in cacti microprop. What is out there is usually not reproducible, important info isconvieniently left out of the methodology.
I have some journal studies on the subject of cacti microp |
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