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 Gr0wer
cultivator &mycologist
Registered: 09/16/03
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So una this is grown on an agar based gel? Do you make it from scratch or buy and custom mix it?
Do you think the liquid cloning gel would work just as well?
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Hooty
Reality isRelative
Registered: 02/24/03
Posts: 2,466
Last seen: 7 days, 1 hour
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 Re: Cactus tissue culture. [Re: Gr0wer]
#2946839 - 07/30/04 11:32 PM (4 years, 5 months ago) |
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I think it's very interesting your work with salvia tissue cultures, but exactly what are the benifits (concidering salvias well document ease of rooting cuttings in nothing but water) of tissue culturing other than just interest?
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neuro
Phytophiliac
Registered: 08/10/99
Posts: 6,514
Loc: Rigel 7
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Re: Cactus tissue culture. [Re: faslimy]
#2946884 - 07/30/04 11:48 PM (4 years, 5 months ago) |
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>>There is another group of people who decided to keep their findings to themselves, they enslaved humanity for world domination. Why keep everyone in the dark on the best methods for things?
That's how it is in the culture industry.
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faslimy
Dead Man
Registered: 04/04/04
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Re: Cactus tissue culture. [Re: neuro]
#2947233 - 07/31/04 01:25 AM (4 years, 5 months ago) |
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Yes, that's also how it is in any industry. It sickens me to think that cultivated hyrids and techniques can never be available to hobbyist growers. Who simply cannot afford to have the resources to experiment so thoroughly, because of this thing called profit which they have put on it.
However, thankyou for sharing those pictures.
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YSr
Registered: 04/13/04
Posts: 81
Last seen: 2 years, 5 months
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Re: Cactus tissue culture. [Re: Una]
#2955090 - 08/02/04 04:42 AM (4 years, 5 months ago) |
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Una, do you recomend any good books?
YSr
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 Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: faslimy]
#2955136 - 08/02/04 05:08 AM (4 years, 5 months ago) |
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I've had many requests of people that want the recipe for the tissue culture of Salvia. Still, this is one thing i want to keep for myself.
Anyone that has basic knowledge of plant tissue culture will probably figure the recipe out in a few months trying. It's not a fancy thing with exotic ingredients.
Although i see your point of view (all information should be free), we live in a world where this is not the case.
The whole tissue culture thing was expensive to start with. We spend at least 2000 euro on equipment, chemicals and apparatus before we had our first plantlet in agar. Even then it took almost a year before we had a devent recipe/protocol.
Can you imagine that i don't want someone to just runaway with the recipe just like that?
On top of that i think, but i may be wrong here, that most of the people who ask me about the recipe, no little or nothing about tissue culture and would be better of asking for a good book than asking for the recipe.
YSr here you go:
Experiments in Plant Tissue Culture by John H. Dodds, Lorin W. Roberts
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Mystiq_Shaman
x.o.
Registered: 07/10/04
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Re: Cactus tissue culture. [Re: Una]
#2955145 - 08/02/04 05:14 AM (4 years, 5 months ago) |
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from what you are saying the information to make such a recipe is availble elsewhere so it is not like you are "hiding" new information but are only protecting your own investment.:)
I found this post very intresting. keep on the good work una
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neuro
Phytophiliac
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Re: Cactus tissue culture. [Re: Una]
#2955389 - 08/02/04 08:20 AM (4 years, 5 months ago) |
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Una, have you thought about selling pre-mixed bags of agar and hormone? I'd suspect you wouldn't have to label the ratios of hormones. I could debate myself either way on this whether it'd be a beneficial thing/novelty for ameture hobbyists (referring to people knowing little or nothing about phytoculture) but at the same time it could be a decent money maker, if only for a while supposing people still can't culture with pre-made mixes.
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Openminded
Dicotyledon
Registered: 08/28/03
Posts: 657
Loc: England.
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Re: Cactus tissue culture. [Re: neuro]
#2955410 - 08/02/04 08:29 AM (4 years, 5 months ago) |
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"We will not be selling plant TC supplies since most of the growth regulators that are needed are toxic (carry the skull icon) and these would require special (expensive) transport."
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YSr
Registered: 04/13/04
Posts: 81
Last seen: 2 years, 5 months
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Re: Cactus tissue culture. [Re: Una]
#2955461 - 08/02/04 08:57 AM (4 years, 5 months ago) |
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neuro
Phytophiliac
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Re: Cactus tissue culture. [Re: Openminded]
#2958048 - 08/02/04 09:04 PM (4 years, 5 months ago) |
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ahh yeah, i had forgotten about that.
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YSr
Registered: 04/13/04
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Re: Cactus tissue culture. [Re: neuro]
#2959100 - 08/03/04 04:58 AM (4 years, 5 months ago) |
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Where do I buy the hormones? Somebody has a link, please?
I just need about 1 gram of each.
YSr
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neuro
Phytophiliac
Registered: 08/10/99
Posts: 6,514
Loc: Rigel 7
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Re: Cactus tissue culture. [Re: YSr]
#2960813 - 08/03/04 03:30 PM (4 years, 5 months ago) |
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Chemical supply companies should have them. Fischer, Sigma and the like, i suspect Fischer and Sigma requires some sort of docs but i doubt all of them do depending on what their stock of chems entails.
Phytotech labs should be good
http://www.phytotechlab.com
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YSr
Registered: 04/13/04
Posts: 81
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Re: Cactus tissue culture. [Re: neuro]
#2963172 - 08/04/04 04:28 AM (4 years, 5 months ago) |
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Una
controlleddemolition
Registered: 03/01/01
Posts: 970
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Re: Cactus tissue culture. [Re: YSr]
#2963820 - 08/04/04 10:57 AM (4 years, 5 months ago) |
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Often used cytokinins are BAP and kinetin
Often used auxins are NAA, IAA and IBA. 2,4-D is not often used because it can cause mutations.
The reason why these are used is because all are autoclavable (although some activity might still be lost) and not too costly. It should be noted that IAA loses it's activity in light in about two weeks.
If you don't have a milligram scale i suggest you buy stock solutions (1mg/ml) and use a pipette or syringe for dosage.
As a basal medium i would suggest Murashige&Skoog or Gamborg B5. I use a mixture that contain macro/micro elements and vitamins.
I assume you have a pH meter because the pH of the medium is very important. You can adjust it with 0,1 M NaOH or HCl.
If i have time i will do a more complete write up about the subject, there's just so much worth mentioning.....
Good luck!
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neuro
Phytophiliac
Registered: 08/10/99
Posts: 6,514
Loc: Rigel 7
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Re: Cactus tissue culture. [Re: Una]
#2966390 - 08/04/04 11:28 PM (4 years, 5 months ago) |
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>>2,4-D is not often used because it can cause mutations.
2,4-D is used as an insecticide because those plants that it kills cannot metabolize that auxin like it can other and natural auxins.
>>there's just so much worth mentioning.....
which is why everyone should get themselves a text book and start reading.
edit: uhh i just noticed this after too day... insecticide = herbicide i'm just stupid and wasn't paying attention to what i was writing.
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Edited by neuro (08/06/04 05:28 PM)
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YSr
Registered: 04/13/04
Posts: 81
Last seen: 2 years, 5 months
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Re: Cactus tissue culture. [Re: neuro]
#2966724 - 08/05/04 02:01 AM (4 years, 5 months ago) |
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I've ordered two of them from Amazon...
YSr
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felixhigh
evil jester
Registered: 06/24/01
Posts: 4,776
Last seen: 2 hours, 40 minutes
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Re: Cactus tissue culture. [Re: YSr]
#2968659 - 08/05/04 03:35 PM (4 years, 5 months ago) |
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knowledge is never too much, i believe! 
FH
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Simisu
taken by gravity
Registered: 08/08/03
Posts: 4,135
Loc: Israel
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Re: Cactus tissue culture. [Re: felixhigh]
#6912794 - 05/14/07 05:09 AM (1 year, 7 months ago) |
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very interesting, thought it could use a 
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Entersandman
Thunderbird
Registered: 06/19/06
Posts: 330
Loc: Non ordinary reality
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Re: Cactus tissue culture. [Re: Simisu]
#6913708 - 05/14/07 11:14 AM (1 year, 7 months ago) |
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nice nice!
i hope to start an equal project in the near future!
where do you get these plant tubes?
http://www.shroomery.org/forums/files/080503-46/72157-pereskiopsis.jpg
...and what are they called?
greetz
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