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OfflineMycologist217
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PF Tek Trial 1: Grow Log-Post Birth-First Flush Harvest- with pics
    #17866420 - 02/25/13 10:19 PM (11 years, 1 month ago)

I started the PF Tek about 2 weeks ago. I used the recommended 90 minute steam sterilization method. I drilled 4 holes in each jar lid and covered them with heavy duty breathable medical cloth tape, and covered each jar with heavy duty aluminum foil prior to steam sterilizing them. I inoculated 12 BRF jars inside of a homemade glove box, and flame sterilized my spore syringe with a homemade alcohol lamp. This set up was in a highly sanitized and disinfected bathroom. After Inoculation i have kept my jars inside an opaque storage bin in a room kept at 80degrees F with a Bionaire space heater with thermostat control.

Since Colonization begun approx 10days ago, all 12 jars have proved contamination free and the fastest of the growers are around 80% colonized.

I plan on giving them the week to consolidate as well as using the dunk&roll.

Below is a picture of my homemade alcohol lamp. As you can see, it is a small mince garlic jar that has been filled with 90% Isopropyl Alcohol. I cut a hole in the lid of the jar, widened it a about 1/4 inch and pulled a stretched out cotton ball through it. It will light after about 5 minutes soaking. Flame is 100% sterile with no residue. 

I know I will see in due time, but can anyone give me an approximation of yield per cake in dry grams?


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Edited by Mycologist217 (03/31/13 07:41 PM)

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Offlinenerdivore
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Re: PF Tek trial 1 [Re: Mycologist217]
    #17866733 - 02/25/13 11:18 PM (11 years, 1 month ago)

2-6 g per cake per flush, more or less depending on your luck with genetics. There's no need to incubate the jars if your room temp is not too cold. And that flame looks a bit excessive for sterilizing a needle...

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OfflineMycologist217
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Re: PF Tek trial 1 [Re: nerdivore]
    #17868522 - 02/26/13 11:23 AM (11 years, 1 month ago)

it might look a bit excessive but as I said...I inoculated 12 jars and came out 100% contam free thus far. I did a bit of research and it appears that using a normal cigarette lighter can (butane) can leave behind a sticky residue on the needle. This isopropyl flame burns very hot and very clean.


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Invisibleslapphappypill
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Re: PF Tek trial 1 [Re: Mycologist217]
    #17868579 - 02/26/13 11:36 AM (11 years, 1 month ago)

well great job on no contams!! Looks like you're well ahead of most first timers :-)

Make sure after you birth, dunk, and roll, that you mist and fan plenty (Fan after misting and fan a lot!) the water droplets evaporating quickly off the cake is a major pinning trigger. If you have enough patience, I'd suggest waiting 2 weeks for consolidation after 100%. Your cakes most likely still wont pin for a good 1-2 weeks if you wait just 1 week of consolidation. Just my 2cents :-)


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I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
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OfflineMycologist217
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Re: PF Tek trial 1 [Re: slapphappypill]
    #17868599 - 02/26/13 11:41 AM (11 years, 1 month ago)

maybe i'll give two of the jars two weeks of consolidation


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Invisibleslapphappypill
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Re: PF Tek trial 1 [Re: Mycologist217]
    #17869042 - 02/26/13 01:05 PM (11 years, 1 month ago)

just remember that the myc spends energy to fruit, and energy to hold of contams, and energy to soak up water.
If you wait the extra time, that gives the myc (cake) a whole extra week to save it's energy up. When you birth your flushes should be better and according to some, possibly more potent


--------------------
We think we have freedom, but we're all just mice in a maze....

                   

FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!

I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~

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OfflineMycologist217
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Re: PF Tek trial 1 [Re: slapphappypill]
    #17870329 - 02/26/13 04:52 PM (11 years, 1 month ago)



if this method was performed in a glove box would it be a very good option?


--------------------
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AMU Q&A-NO SYMPATHY FOR THE DEVIL! KEEP THAT IN MIND! BUY THE TICKET: TAKE THE RIDE

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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.

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OfflinePussyFart
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Re: PF Tek trial 1 [Re: Mycologist217]
    #17870648 - 02/26/13 05:48 PM (11 years, 1 month ago)

Quote:

Mycologist217 said:
if this method was performed in a glove box would it be a very good option?



Not really, he is using WAY too many spores for just a syringe or 2.

Mushrooms are fruited in the open air, this means the print will never be 100% clean.

But we do all we can to prevent adding any more vectors of contaminents by taking the precations we do.

When you make a syringe from a print, you want to take the tiniest amount of spores you can, to minimize the chance of grabbing a contam.

The syringe he just made in that video is heavily contaminated. No gloves, open air, he scraped all the spores/contams from multiple prints, and he used an unsterile scalpel to do it.....Total and utter fail.

Edited by PussyFart (02/26/13 05:49 PM)

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Offlineveda_sticks
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Re: PF Tek trial 1 [Re: Mycologist217]
    #17870773 - 02/26/13 06:13 PM (11 years, 1 month ago)

no need to keep your jars in a sealed storage box.

you only need to be carefull of contamination in the inoculation procedure,. once thats done you can keep you jars anywhere. since have a filter/ (the dry verm layer)


Quote:

Mycologist217 said:
maybe i'll give two of the jars two weeks of consolidation




2 weeks is a bit excessive, my jars usually pin pretty quick after a week. normalyh within a few days.


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OfflineMycologist217
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Re: PF Tek trial 1 [Re: veda_sticks]
    #17870886 - 02/26/13 06:33 PM (11 years, 1 month ago)

my main area of interest at this point is selecting the best way to go about repeating this project without buying fresh new spore syringes again. What is the generally accepted best way?


--------------------
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OfflinePussyFart
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Re: PF Tek trial 1 [Re: Mycologist217]
    #17871375 - 02/26/13 08:05 PM (11 years, 1 month ago)

Quote:

Mycologist217 said:
my main area of interest at this point is selecting the best way to go about repeating this project without buying fresh new spore syringes again. What is the generally accepted best way?



This can be done in a SAB.

http://www.mushroomvideos.com/Sporeprinting-Syringes

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OfflineMycologist217
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Re: PF Tek trial 1 [Re: PussyFart]
    #17871493 - 02/26/13 08:28 PM (11 years, 1 month ago)

Day 11 after inoculation: Everything still appearing contamination free. For the past few days there has been water droplets condensed inside of the jars. No negative effects noticed. The fastest colonizers of my 12 jar project are ~90% while the slowest colonizers are ~60%. Ideally speaking, I'd like to birth all 12 at the same time so as to create the proper micro-ecosystem indicated by the PF Tek. Is there reason to not let the fastest growers consolidate until the slowest growers are a week consolidated?


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OfflinePussyFart
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Re: PF Tek trial 1 [Re: Mycologist217]
    #17871515 - 02/26/13 08:31 PM (11 years, 1 month ago)

No, let them consolidate a little longer, they will be fine.

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Re: PF Tek trial 1 [Re: slapphappypill]
    #17871541 - 02/26/13 08:38 PM (11 years, 1 month ago)

Quote:

slapphappypill said:
just remember that the myc spends energy to fruit, and energy to hold of contams, and energy to soak up water.
If you wait the extra time, that gives the myc (cake) a whole extra week to save it's energy up. When you birth your flushes should be better and according to some, possibly more potent




I too am on my very first cultivation grow so this thread caught my eye instantly. I'm behind the OP having only inoculated a day ago. This advice about waiting 2 weeks after 100% colonization is pure genius!!! I'm learning so friggen much from these threads and this forum site...so cool!!!

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OfflineMycologist217
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Re: PF Tek trial 1 [Re: BovineScattologist]
    #17871545 - 02/26/13 08:39 PM (11 years, 1 month ago)

well shoot, feel free to document your project on this thread too, and let's keep generating knowledge for us newbies. Are you using the Pf Tek?


--------------------
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Re: PF Tek trial 1 [Re: Mycologist217]
    #17871616 - 02/26/13 08:52 PM (11 years, 1 month ago)

Quote:

Mycologist217 said:
well shoot, feel free to document your project on this thread too, and let's keep generating knowledge for us newbies. Are you using the Pf Tek?




That sounds like a good idea. I am using the BRF pf tek. Actually I'm following Shea25's tek here: Shea25's pf tek

I did everything you mentioned except I used a three flame cigar lighter for sterilization and I didn't cover the holes on my jars. In the tek from Shea25 he said this:

" I have done TONS of PF's and the dry verm barrier has not let me down. You will see. I have told so many people when there asking why are my jars going so slow and it because people cover the holes. Theres just no need to, or whats the point of the dry verm? "

So, we'll see...

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OfflineMycologist217
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SGFC [Re: Mycologist217]
    #17871625 - 02/26/13 08:54 PM (11 years, 1 month ago)

As per the PF Tek, I have constructed a Shotgun Fruiting Chamber (SGFC). I wonder if should filled it with the soaked perlite at the point when by jars start consolidating so that the humidity in the FC builds up and is ready for the cakes at birth?


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Mycologist217 is a fictitious entity that uses images supplied by Google to mask his/her inability to develop normal social habits.

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OfflineMycologist217
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Re: PF Tek trial 1 [Re: BovineScattologist]
    #17871630 - 02/26/13 08:56 PM (11 years, 1 month ago)

I've also read that the tape is just a "security blanket". But at this point I have a whole roll of it, and it hasn't let me down yet!


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Re: SGFC [Re: Mycologist217]
    #17871639 - 02/26/13 08:57 PM (11 years, 1 month ago)

sure y not better to see everything working out beforehand just in case right


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Re: SGFC [Re: cronicr]
    #17871770 - 02/26/13 09:22 PM (11 years, 1 month ago)

may i post my thread to im going to inoculate today 2nd timer
ny first tike i got 1 out of 6 so i am major newbie


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