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 shirley knott
not my real name
Registered: 11/11/02
Posts: 9,000
Loc: London
Last seen: 5 months, 7 days
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 pan cyan first try (failed)
#1700148 - 07/09/03 03:52 PM (5 years, 4 months ago) |
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This is my first try with a species of fungus other than ps. cubensis. if it's successful, i'm away to try plenty of others. wish me luck 
19 June - poured 5 half-jarsfuls of dry wild birdseed into pillowcase. soaked pillowcase in saucepan of water. changed water three times altogether. soaked 24h. then hung pillowcase up to drain (corner downwards) for 2 hours, so there's no dripping at all.
20 June - loaded wbs into jars - now each approx two-thirds-full. put square of tyvek on top, and added lids (one half-inch hole and one teeny injection hole, though i'm not using the injection hole this time, as i have agar from a friend). loaded into pressure cooker, and cooked 15psi for 90 mins. cooled overnight, in the unopened pc.
24 June - i'd been supplied with cyan mycelium on an agar plate, and agar pieces in ziplock bags: cleaned hands, donned mask, cleaned jars, loosened jar lids, heated then swabbed tweezers, opened myc bag, removed lid, tweezed out myc on agar sliver, flicked / knocked into jar, replaced lid. repeated x 4. total 5 jars inoculated with panaeolus cyanascens.
28 June - jars growing I think (?) ? looks like the kind of thing I?d have chucked if this was cubensis: tiny white dots, thickening slightly but still new to me??. I expect it to turn green at any moment!
2 July ? 3 jars ? contaminated, chucked out: - probable wet spot?
  
3 July - 3 new jarfuls soaked, pced, inoculated more carefully, with pieces freshly cut from the plate this time.
9 July - good news - the new ones (and 2 of the originals) are now growing along nicely. here they are today. you can see a sliver of agar in the top pic:
 
that's it so far. sorry to leave you high and dry without fruit shots. i plan to wait until a week after full colonisation, then pasteurise some straw/poo (90%:10%) and spawn it in. 
will post updates. sorry to put a pic on each line, i'll redo it if the codemasters fix a software bug that means i have no choice.
sk 
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motaman
old hand
Registered: 12/18/02
Posts: 5,024
Last seen: 2 years, 26 days
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Good Luck.. I can't wait to see your progress. 
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 angryshroom
Registered: 12/18/01
Posts: 7,253
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Geez, that mycelium looks really white!
In all my experiments with Panaeolus species, I've found that the mycelium is more gray and whispy, looks somewhat similar to cobweb.
I've had bad experiences with shaking the this mycelium as well. I wouldn't recommend that. It will die and contaminate.
Very good luck to you shirley. 
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afoaf
CEO DBK?
Registered: 11/08/02
Posts: 32,305
Loc: Ripple's Heart
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you cannot fail, you're blessed.
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Raadt
nicht
Registered: 06/07/02
Posts: 2,104
Loc: azurescending
Last seen: 10 months, 13 days
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air exchange, and temp.
you will likely need to heat your fruiting chamber. to 88deg or so.
and cube air exchange is NOT sufficient.
you will definantly need to fan very often, or automate.
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Raadt
-- The information I provide is only information from readings, growing of gourmet mushrooms, and second hand stories--
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angryshroom
Registered: 12/18/01
Posts: 7,253
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 Re: pan cyan growlog [Re: Raadt]
#1704647 - 07/11/03 12:37 AM (5 years, 4 months ago) |
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You would say that fruiting temps are in the high 80's!? Whoa.
Doesn't the mushroom cultivator say high 70's?
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Paid
Pict
Registered: 03/12/03
Posts: 5,376
Loc: Zone ate
Last seen: 4 years, 9 months
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Good luck Shirl :-)
Looking good so far mate.
You always have to sleep on the wet spot 
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m0rb
mushroom mobster
Registered: 05/07/03
Posts: 184
Loc: New England
Last seen: 4 years, 4 months
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 Re: pan cyan growlog [Re: Paid]
#1705025 - 07/11/03 02:58 AM (5 years, 4 months ago) |
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Good luck shirley! My friend has followed your wonderful posts in the past, and thanks you for all the great contributions you have made to this community. You are truely amazing sometimes with what information you give back to others. Eric and that monkey of yours must be some lucky animals...and um.. people too! hehehe
Cant wait to see how this turns out. I will eagerly be checking back daily like a little kid at the toy store!
-m0rb-
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"The business of America is business," - Calvin Coolidge
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Jackal
Well Versed InEtiquette
Registered: 10/16/02
Posts: 4,528
Last seen: 1 year, 20 days
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 Re: pan cyan growlog [Re: m0rb]
#1705060 - 07/11/03 03:32 AM (5 years, 4 months ago) |
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Yeah, hope this works for you. I really wanted to try Pans, but mine contammed. Fingers crossed. 
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ralphster44
Vendor/collector
Registered: 01/03/01
Posts: 4,240
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Re: pan cyan growlog [Re: Jackal]
#1706065 - 07/11/03 01:34 PM (5 years, 4 months ago) |
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Pans are the best....
Your doing good so far:)
A little lime and dung goes a long way.
Cant wait to see your success
Ralphster44
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WE SHIP TO CANADA FROM WITHIN CANADA
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shirley knott
not my real name
Registered: 11/11/02
Posts: 9,000
Loc: London
Last seen: 5 months, 7 days
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 Re: pan cyan growlog [Re: m0rb]
#1706662 - 07/11/03 05:21 PM (5 years, 4 months ago) |
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thank you all for your too-kind and unwarranted words of encouragement (/me does a midair backflip and flushes red)
i'm a newbie too, i just love this hobby to pieces, and get a real buzz from sharing my newly-learned tips, and showing off my successes
i have plenty failures, too, but i don't post those so often  always happy to help if i can, on the boards or by pm.
i feel compelled to give updates even when not much is happening, so here's the first:
1. 2 jars, inoculated from dodgy wedges 24/06: 
the one on the right was shaken, and never recovered. lesson learned #1 ! still not sure if these will progress to full colonisation.
2. 3 jars, inoculated from fresh plate wedges 3/07:  these look like they'll make it to stage 2
3. a cubensis jar (creeper) at 99%, for comparison:  - whiter, thicker, more rhizomorphic (rope-like tendrils)
i'm left a little confused as to fruiting temps, though that is still a way off. i had thought that 75-80 would be sufficient. i also have no idea as to any differences expected in spawn run time, prepinning time etc, but what the hell: THAT is the funnest part of this hobby, the steep bit of the learning curve when it's all new and exciting.
sk
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tastyshroom
Mr.Shroom
Registered: 03/15/03
Posts: 820
Loc: Washington
Last seen: 1 year, 1 month
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Looks good
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Perspective on life always changes
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m0rb
mushroom mobster
Registered: 05/07/03
Posts: 184
Loc: New England
Last seen: 4 years, 4 months
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ooh man! the suspense! aaaaah!
-m0rb-
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"The business of America is business," - Calvin Coolidge
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shirley knott
not my real name
Registered: 11/11/02
Posts: 9,000
Loc: London
Last seen: 5 months, 7 days
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 pan cyan growlog - ?lipstick mould? [Re: m0rb]
#1732209 - 07/20/03 12:06 PM (5 years, 4 months ago) |
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20 july update - the 2 jars (inoculated 26 june, one shaken) are continuing as they were. the shaken one looks stalled and i've given up on it. the other one continues to advance. i think the wbs was a little dry, hence the slow progress.
>>>>>>>>>>>
the 3 others (inoculated 3 july) are munching slowly: 
if they were cubes i'd shake to speed em up, but i'm gonna just wait it out. however, is this lipstick mould i'm seeing? all three opf these have a pale pinkish tinge. could it be from the 'red bombs' (red millet) that fugu talks about, or does it look bad?
it's a very pale pink, but it's pink nonetheless - more so than comes out in these crappy webcam pictures. i'm gonna stick these last two in the contam forum, in case it triggers somebody's memory there too.
sk 
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scotsman1
addict
Registered: 06/24/02
Posts: 804
Loc: guess
Last seen: 1 month, 28 days
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Re: pan cyan growlog - ?lipstick mould? [Re: shirley knott]
#1732225 - 07/20/03 12:15 PM (5 years, 4 months ago) |
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LOOKING GOOD
i was once told to remove
the black seeds from the mix
sunflower seeds if i remember
correctly
i cant remember the reason though
you'd need to see Hip about that
but your is looking good
your leaving your footprint in the
mushroom community
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Cow Shit Collector
Patty Poacher
Registered: 02/14/01
Posts: 1,959
Loc: Random Field
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Re: pan cyan growlog - ?lipstick mould? [Re: scotsman1]
#1733914 - 07/21/03 01:12 AM (5 years, 4 months ago) |
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I've seen a similar PINK experience with pans in birdseed. I wasn't sure what it was so I threw them out. get a more experienced answer before you do so. I was told it was a nasty bacteria. Anything that stains pink has to be pretty bad, but I think your right on the money with the dye or the colors comming out.
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recalcitrant
My Own God
Registered: 04/20/02
Posts: 2,927
Loc: Canada West
Last seen: 6 months, 28 days
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Re: pan cyan growlog - ?lipstick mould? [Re: scotsman1]
#1734722 - 07/21/03 11:25 AM (5 years, 4 months ago) |
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I'd like to know why you take out the sunflower seeds in WBS.
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We have to answer our own prayers
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vampirism
Whiskey Mystic
Registered: 03/14/04
Posts: 8,093
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Re: pan cyan growlog - ?lipstick mould? [Re: recalcitrant]
#1736121 - 07/21/03 07:00 PM (5 years, 4 months ago) |
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harder to sterilize, high fat content and low nutritional value for the fungi.. its waste that also attracts bacteria- i made the mistake of using it alone one time =\
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preschooler
new hand
Registered: 01/27/03
Posts: 1,952
Loc: wouldn't you like to know...
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Re: pan cyan growlog - ?lipstick mould? [Re: ]
#1736200 - 07/21/03 07:38 PM (5 years, 4 months ago) |
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im a newbie and i probably would just screw up a pan grow. But wouldn't you think rye berries would work well with the pans? the suspense of this grow is killing me. i wish joushua would try these too.
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scotsman1
addict
Registered: 06/24/02
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Re: pan cyan growlog - ?lipstick mould? [Re: ]
#1737935 - 07/22/03 10:52 AM (5 years, 4 months ago) |
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Quote:
Morrowind said:
harder to sterilize, high fat content and low nutritional value for the fungi.. its waste that also attracts bacteria- i made the mistake of using it alone one time =\
i new there was a good reason for it
take no chances
cut no corners
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