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 chefinainteasy
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Registered: 12/13/10
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 Pins on pins??? 
#15865282 - 02/26/12 10:20 AM (1 year, 2 months ago) |
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Well these bags seemed to be doing fine. Started knotting in incubation. Put into the fruiting conditions, 95%RH, FAE 45 min out of 60. Good light. But the fruits have seemed to stall, and now have an explosion of new pins everywhere in the bags, even on the already forming fruits. These are grey oysters cultured from spores, on agar, then to grain, then pasteurized straw. Is this just a poor preforming strain i have isolated? Should i reduce the RH? Any advice?
   
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RogerRabbit
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Quote:
chefinainteasy said:
It means they don't like your substrate for whatever reason. The original fruits couldn't finish growing on it and stalled. The new ones found the old fruits better suited than your straw.
RR
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Mycelio
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Chefin, are you sure about constant 95%RH? Those shriveled caps on pic 3 and 4 appear to have dried out at some point.
Carsten
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chefinainteasy
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Re: Pins on pins??? [Re: Mycelio]
#15866013 - 02/26/12 03:08 PM (1 year, 2 months ago) |
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I have a humidistat set to 95% but when the fan outside the GH runs the RH drops a bit, maybe 3% when the humidifiers kick back on. I usually have a separate humidity gauge besides the humidistat just to check the accuracy. Im not sure what would be wrong with the straw. I have had success with the same product and procedure, but not this strain of mushroom. So i guess these are a lost cause then?
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Mycelio
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I wouldn't give up on them yet. Another possibility could be excessive CO2 levels. Am I right, assuming this is in an indoor greenhouse, which recently got crowded by substrate blocks?
Carsten
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chefinainteasy
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Re: Pins on pins??? [Re: Mycelio]
#15867106 - 02/26/12 07:55 PM (1 year, 2 months ago) |
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Yes it is indoor. Wouldn't call it crowded in there yet but is definitely more full that it has been. Would you suggest more FAE then? I have slits all over the gh and a box fan that runs often towards the bottom of the gh to expel co2. Plus two comP fans that run with slits behind them to draw in fresh air. Should I try cut tin the bag off of one or two of them?
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Mycelio
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The funnel shaped oyster caps you have grown before, already indicate high CO2 levels, which must be even higher now. All those pins on and between the stems of aborted mushrooms remind me of the typical coral growth, caused by too much CO2.
Increasing air exchange with the room won't help much, you need to get fresh outside air into the greenhouse.
Carsten
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chefinainteasy
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Re: Pins on pins??? [Re: Mycelio]
#15869437 - 02/27/12 09:44 AM (1 year, 2 months ago) |
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Oh ok i see what you are saying. Even though im drawing air out of the greenhouse the air in the basement isn't enough to count as fresh air. I guess its time to set up my air intake from the basement windows.
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chefinainteasy
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So here is what i came up with. Its just some blue board insulation with a 140mm computer fan positioned to draw air in. I taped the flexible ducting to it and ran it to the top of the greenhouse so the air comes in right above the rack with the bags on it. It turns on with the humidifiers and other computer fans about two or three times an hour for about 5-10 min each time depending on the humidistat. Then there is still the box fan that draws air out on a timer for 45-30 min/hr. Hopefully this does the trick.
  
Thats only if its a CO2 issue. If its a substrate or culture issue, i guess the FAE will help but wont change much. Here are some pics from today. I picked off some of the aborted clusters. I left some of the pin on pin clusters to see what comes of them. A few clusters look ok, but definitely not proper formation.
  
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Mycelio
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Oysters are fresh air junkies, so this should definitely improve things, but looking at the better developing clusters I doubt that CO2 was the only problem. Right now I have no other idea. Light must be fine, temperature can't be too high, substrate moisture shouldn't be too low. Let's see how the new pins develop.
And be warned, at some point you will want to install a second pipe, blowing spore filled air from the greenhouse outside.
Carsten
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mikesethnobotany
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Re: Pins on pins??? [Re: Mycelio]
#15871440 - 02/27/12 05:50 PM (1 year, 2 months ago) |
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New pictures are looking great man! Keep up the great work, you'll get it all dialed in with time.
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chefinainteasy
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Thanks guys. I have thought about exhausting air out when i actually am able to produce a decent quantity. I just found my two king oyster grain bags fully colonized but contaminated, not cool. I ordered 4# of grey oyster spawn from field and forest out of frustration with my grain bag experiences. So hopefully i will be able to test out the GH with a reliable fruiting strain.
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Check out my youtube videos. the Mushrooms Naturally series by chefinainteasy
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chefinainteasy
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So here are the results of more FAE and also an increase in humidity. Turns out my humidistat was about 7-10% off. I had it set to 95% but on others suspicions that humidity was low, put a few hygrometers to check the calibration. Turns out it was actually around 85-87%. So i switched to a timer to run my humidifiers and now it stays between 96-99%. By no means the best fruits i have produced but at least im realizing my conditions were off and rectifying the issue before my larger batches were put into fruiting conditions and ruin them. Here are a few pics in the improved conditions.
   
Here are some of the larger fruits i picked this morning. They are very thin and scrawny. Maybe just the culture i isolated isn't a very good producer? Oh well, on to the next.
  
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Check out my youtube videos. the Mushrooms Naturally series by chefinainteasy
My new business is now on facebook at http://www.facebook.com/#!/MushroomsNaturally
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