Do I need to quickly toss my agar if contaminates pop up? - Mushroom Cultivation

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Big_Sexy
LoveMonger

Registered: 10/04/11
Posts: 602
Last seen: 11 years, 3 months

Do I need to quickly toss my agar if contaminates pop up?

#15749194 - 02/01/12 08:48 PM (12 years, 2 months ago)

Do I need to quickly toss my agar if contaminates pop up? I know you should never open a contaminated agar plate in a flow hood but do I need to toss the agar plates that have trich. on them (1 of my plates has trich.) quickly, If they stay around is there a risk of the spores or whatever spreading or can you isolate away from trich. without spreading it? I know you can isolate away from contaminates but I was thinking trich. might be different since it seems to be the most prevalent.

Please and thanks

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Swwert
Truck-Boat-Truck

Registered: 04/08/11
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Re: Do I need to quickly toss my agar if contaminates pop up?

[Re: Big_Sexy]
#15749257 - 02/01/12 09:04 PM (12 years, 2 months ago)

why would you want to keep it around?

just curious

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Shamrocker
mst3k

Registered: 02/17/11
Posts: 385
Last seen: 11 years, 11 months

Re: Do I need to quickly toss my agar if contaminates pop up?

[Re: Swwert]
#15749337 - 02/01/12 09:21 PM (12 years, 2 months ago)

I haven't gotten into agar yet, but I believe one of it's advantages is you can isolate good myc from contams. However I'd think mean green would spread spores from a petri dish just as it would from a cake or tub. I'd toss it.

Good luck!

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Big_Sexy
LoveMonger

Registered: 10/04/11
Posts: 602
Last seen: 11 years, 3 months

Re: Do I need to quickly toss my agar if contaminates pop up?

[Re: Swwert]
#15749461 - 02/01/12 09:44 PM (12 years, 2 months ago)

Quote:

Swwert said:
why would you want to keep it around?

just curious

To keep an eye out for extremely rhizomorphic mycelium, I have 2 trays with 3 sample apiece so I wanted to possibly keep them so if i see some really good looking myc I wanted to isolate it onto another plate, I have a limited number of plates or else I would just toss it!

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HorizonSpawn
Gettin' my grow on :)

Registered: 05/14/11
Posts: 1,739
Loc: Myco-Tek.org

Re: Do I need to quickly toss my agar if contaminates pop up?

[Re: Big_Sexy]
#15749513 - 02/01/12 09:51 PM (12 years, 2 months ago)

If the trichoderma has yet to sporulate, then you should be fine to transfer away from it into a new plate...

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Big_Sexy
LoveMonger

Registered: 10/04/11
Posts: 602
Last seen: 11 years, 3 months

Re: Do I need to quickly toss my agar if contaminates pop up?

[Re: HorizonSpawn]
#15749548 - 02/01/12 10:00 PM (12 years, 2 months ago)

How do i tell when it sporulates?

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HorizonSpawn
Gettin' my grow on :)

Registered: 05/14/11
Posts: 1,739
Loc: Myco-Tek.org

Re: Do I need to quickly toss my agar if contaminates pop up?

[Re: Big_Sexy]
#15749578 - 02/01/12 10:05 PM (12 years, 2 months ago)

The spores of trichoderma are green... It's mycelium is a dense bright white.

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Mushrooms, Mycology and Psychedelics > Mushroom Cultivation

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