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I use the open air, self-healing injection port method of inoculation. I've been trying to think of whether or not there is any truly feasible way of contamination from getting into jars made in this fashion with synthetic filter discs for gas exchange. I believe if you're flame sterilizing the needle, the only real point at which spores could possibly vector their way in would be in the motion from the flame to the injection port, after the needle has cooled a bit below glowing red hot.
I typically flame till glowing bright red, shoot a bit of spore solution out to cool the needle, and the moment the liquid isn't sizzling on the way out I plunge the needle into the injection port.
I haven't done a ton of jars yet (about two dozen) but so far no contamination. I do think I recently had some cobweb spores from a test jar (I let it get exposed on purpose to learn about contams) get out from the jar, so I'm going to have to sterilize and clean up my work area, but there's no carpet, so.
-------------------- <metatron> i dont think the surface area of a mushroom is quite ideal to administer psychoactive compounds anally
Quote: Cubensis said: ...could any harm come of cooling the red hot needle by dipping it in rubbing alcohol (instead of wasting spore solution to cool it down?)
Yes. Alcohol is not sterile, its aseptic. Alcohol does not sterilize. The small amount of spore solution that is used to cool that needle back down will become insignificant after you print your first caps. The key is to do that first grow correctly and without contamination. If you do, you'll have more spores than you know what to do with.