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 EvilMushroom666
Heretic
 
Registered: 11/18/09
Posts: 10,114
Loc: Canada 
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 Flame Sterilization Experiment  3
#15186837 - 10/06/11 04:14 PM (1 year, 8 months ago) |
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After hearing people bicker back and forth for so long I have made the decision
to make a visual experiment to see the difference between flame sterilizing
and flame sterilizing then wiping with an ISO swab. This experiment
was done in front of a laminar flow hood.
I have six agar plates, marked A, B, C, D, E & F that I will use for this experiment:
Plate A:
Scalpel was flame sterilized until red hot and then allowed to cool before
making 15 slash's in the plate both vertically and horizontally.
Plate was then sealed with parafilm.
Plate B:
Scalpel was flame sterilized until red hot, and then was wiped down immediately
after with an 70% ISO alc swab. After the wipe down I took plate B and
made 15 slash's in the plate both vertically and horizontally.
Plate was then sealed with parafilm.
Plate C:
18G needle was removed from package and in open air flame sterilized.After
being sterilized in open air the needle was moved to the flow hood and
75 holes were poked into the agar plate with that needle.
Plate D:
18G needle was removed from package and in open air flame sterilized
and then wiped down with a 70% ISO sawb. After being wiped down in open
air the needle was moved to the flow hood and 75 holes were poked into the
agar plate with the needle.
Plate E:
Scalpel was wiped down with 70% ISO swap in open air.Then the scalpel
was moved to the hood and 15 slash's were then made into the agar
plate both horizontally and vertically.
Plate F:
Needle was wiped down with 70% ISO swab in open air. Then the needle
was moved to the hood and 75 holes were poked into the agar plate with
the needle.
I will be updating this post with the results in a few days regardless
of what they show to once and for all settle this topic.
Edited by EvilMushroom666 (10/06/11 06:24 PM)
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TranscendingLife
I Don't Need a Life to Live
Registered: 06/09/10
Posts: 20,825
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Looking for Dictyophora Indusiata (Phallus Indusiatus) Cultures Please PM me for a trade
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
   How I Do EVERYTHING
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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 domesticgnome
Registered: 04/22/11
Posts: 894
Loc: For me to know and you to...
Last seen: 1 hour, 3 minutes
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Cool beans. Can't wait.
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Wishyouwanna
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The Influence
I make it do what it do
Registered: 03/30/11
Posts: 4,387
Loc: Not Wisconsin
Last seen: 1 year, 11 days
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Bad: Waking up to find a penis has been drawn on your face. Worse: Finding out it was traced 
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Munchauzen
Steal Your Shrooms
Registered: 06/23/11
Posts: 842
Loc: Mid-X
Last seen: 7 months, 3 days
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400 on redhot to winout the series
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TranscendingLife
I Don't Need a Life to Live
Registered: 06/09/10
Posts: 20,825
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Re: Flame Sterilization Experiment [Re: Munchauzen]
#15187052 - 10/06/11 04:58 PM (1 year, 8 months ago) |
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Quote:
Munchauzen said:
400 on redhot to winout the series
I'll take that bet, as all 4 are redhot.
I got 1000 on all 4 are the same.
--------------------
Looking for Dictyophora Indusiata (Phallus Indusiatus) Cultures Please PM me for a trade
AMU: We Quickly Answer Questions Here
"One must accept the probability of failure to experience the elation of success." - TranscendingLife
“A man of genius makes no mistakes; his errors are volitional and are the portals of discovery.” - James Joyce
How I Do EVERYTHING
"Your vision will become clear only when you can look into your own heart…. Who looks outside, dreams; who looks inside, awakes."- Carl Jung
"Anything that can be done chemically can be done by other means."- William S. Burroughs
"You are as dead now as you will ever be" - Seth
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SKrink
KING MOB
Registered: 01/29/11
Posts: 1,042
Last seen: 4 months, 2 hours
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Quote:
EvilMushroom666 said:
After hearing people bicker back and forth for so long I have made the decision
to make a visual experiment to see the difference between flame sterilizing
and flame sterilizing then wiping with an ISO swab.
The only way to settle an argument! THANK YOU! Eagerly awaiting the results, whatever they are.
This could be the start of a Shroomery FACT OR FICTION series.
(mythbusters sounds much catchier, but I doubt the intention is busting myths.)
--------------------
 SWEET POTATO HOME FRIES
 HOW TO USE A PENIS ENVY SPORE SWAB
  
... Oh mighty masticator, salivator, vocalizer, swallower, licker biter sucker brow-knitter looker blinker rubbernecker thumber prodder up-yours fingerer ringwearer nosepicker waver drinker armlifter bodybender hipswiveler kneer springer runner ZERO::::::::OOOOOOOOO:::::::: RUN!!!
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,114
Loc: Canada
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Re: Flame Sterilization Experiment [Re: SKrink]
#15187075 - 10/06/11 05:06 PM (1 year, 8 months ago) |
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Just figured I would toss together a rough demonstration/experiment to
see what happens. Before any of the more scientific minded individuals
mention that I do not have a control, note that the plates were made using
my hood and therefore I know my HEPA is flowing sterile air.
I honestly do not know what to expect, and obviously doing 4 petri dish's
is not likely to provide concrete evidence that can be written in stone,
but either way it may provide some food for thought.
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Twistedd
into a knot of dichotomies.
Registered: 09/26/11
Posts: 108
Loc: On the Fence.
Last seen: 3 days, 16 hours
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Re: Flame Sterilization Experiment [Re: SKrink]
#15187082 - 10/06/11 05:06 PM (1 year, 8 months ago) |
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Sea_Goat
Strangest
Registered: 06/25/11
Posts: 143
Last seen: 1 year, 8 months
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Re: Flame Sterilization Experiment [Re: Twistedd]
#15187255 - 10/06/11 05:51 PM (1 year, 8 months ago) |
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"Disinfectants are substances that are applied to non-living objects to destroy microorganisms that are living on the objects.[1] Disinfection does not necessarily kill all microorganisms, especially nonresistant bacterial spores; it is less effective than sterilization, which is an extreme physical and/or chemical process that kills all types of life."
Alcohol is a disinfectant.
A red hot needle or scalpel is over 1000 degrees Fahrenheit which is plenty hot enough to sterilize.
Why would anyone want to disinfect a sterile object?
I hope the alcohol wiped ones fail so those less informed can see for themselves, but I'm guessing there wont be much difference in the dishes.
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brand0n
large jar mad man!!
Registered: 02/09/09
Posts: 842
Last seen: 1 month, 23 days
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Re: Flame Sterilization Experiment [Re: Sea_Goat]
#15187271 - 10/06/11 05:54 PM (1 year, 8 months ago) |
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ya im gonna bet there isnt a diff what so ever.
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That did it! I am now the anti BRF CAKE! I have had enough! TO HELL WITH BRF! REAL MEN USE SEED! Go now brf younging. Get some seed. COME BACK A MAN!
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The Influence
I make it do what it do
Registered: 03/30/11
Posts: 4,387
Loc: Not Wisconsin
Last seen: 1 year, 11 days
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Re: Flame Sterilization Experiment [Re: Sea_Goat]
#15187279 - 10/06/11 05:55 PM (1 year, 8 months ago) |
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OP you should do some with just alcohol swabbed syringes/scapels, I see a lot of posts were people say that is suffcient.
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Bad: Waking up to find a penis has been drawn on your face. Worse: Finding out it was traced
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,114
Loc: Canada
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Quote:
The Influence said:
OP you should do some with just alcohol swabbed syringes/scapels, I see a lot of posts were people say that is suffcient.
Let it be so.
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Sea_Goat
Strangest
Registered: 06/25/11
Posts: 143
Last seen: 1 year, 8 months
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Quote:
EvilMushroom666 said:
Let it be so.
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Wing
The Eye Tyrant
Registered: 04/26/11
Posts: 2,802
Loc: Middle of the East Coast 
Last seen: 4 months, 10 days
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Awesome idea EM! Can't wait to see the results
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My Grow Logs
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,114
Loc: Canada
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Re: Flame Sterilization Experiment [Re: Sea_Goat]
#15187335 - 10/06/11 06:06 PM (1 year, 8 months ago) |
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Added plates E & F to original post, will be making them a bit later tonight.
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igigi
Registered: 09/10/11
Posts: 154
Loc: Sumer, Mesopotamia
Last seen: 1 month, 27 days
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Quote:
TranscendingLife said:
NICE experiment, bro!
--------------------
I --REFUSE-- TO BELIEVE Boeing 767s and WTCs are made out of exploded and unexploded nano-thermitic material.
Google: bentham science thermitic. Click and READ the first link. I triple (WTCs 1, 2, and 7..) dog dare you.
http://ae911truth.org/
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m_jacobs
Man Whore
Registered: 03/11/11
Posts: 121
Loc: CA
Last seen: 16 days, 28 minutes
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Re: Flame Sterilization Experiment [Re: igigi]
#15187720 - 10/06/11 07:17 PM (1 year, 8 months ago) |
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Did you use a new blade or scalpel on plates A,B and E,F?
Or is it the same scalpel over and over again?
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EvilMushroom666
Heretic
Registered: 11/18/09
Posts: 10,114
Loc: Canada
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Re: Flame Sterilization Experiment [Re: m_jacobs]
#15187737 - 10/06/11 07:20 PM (1 year, 8 months ago) |
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Same blade, but flame sterilized or wiped down depending upon the plate.
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scifipirate
Jug Fuckler
Registered: 01/17/09
Posts: 756
Loc: FL
Last seen: 5 months, 22 days
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good idea
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