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 Lapius
Super Fighting Robot
Registered: 04/05/02
Posts: 308
Loc: Earth 25XX
Last seen: 2 years, 6 days
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 Potato Dextrose Agar 
#4308913 - 06/17/05 06:59 PM (6 years, 11 months ago) |
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Acquired some corn syrup. Had a choice of a few. Chose light corn syrup w/ vanilla. Didn't want the vanilla in it, but than, didn't want dark thick corn syrup, purely for contaminate visibility reasons. Was I wrong to get the clear "light" syrup w/ added vanilla? Should I just acquire the basic dark corn syrup? 
Here is the recipe:
Potato Dextrose (Yeast-extract) Agar (PD(Y)A)
broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)
9 g agar agar
7 grams dextrose( = glucose, or 10 ml honey or corn syrup )
1 gram brewers yeast or yeast-extract (optional)
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 Roadkill
Retired Shroomery Mod
 
Registered: 12/11/01
Posts: 22,580
Loc: Snoqualmie, Wa.
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 Re: Potato Dextrose Agar [Re: Lapius]
#4309483 - 06/17/05 09:34 PM (6 years, 11 months ago) |
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I have never tried using corn syrup with mine.
I do use PDYA and MEA.
I follow pretty much the same recipies as are stated in the FAQ.
Copy and Pasted from the FAQ :
Quote:
These recipes are designed for agar maintenance of Psilocybe species. All media have a near-neutral pH and should be sterilized for twenty to thirty minutes at 15 psi pressure. 500 ml of medium will pour around 20 petri dishes. Using a different medium from time to time will prevent the culture getting used to one medium.
Malt Extract Agar (MEA)
10 grams light malt extract
9 grams agar agar
500 ml potable or distilled water
Potato Dextrose (Yeast-extract) Agar (PD(Y)A)
broth from boiling 150 grams sliced potatoes
in 500ml water for 30 minutes(add water to 500ml)
(You can use 5 g of instant potato flakes instead)
9 g agar agar
7 grams dextrose( = glucose, or 10 ml honey or corn syrup )
1 gram brewers yeast or yeast-extract (optional)
If you read the PDYA one there are several different ways you can make it.
This is the one I make.
-PDYA-
500ml water
5 grams of instant potato flakes(Potato Buds Brand)
9 grams agar agar
10 ml honey (Raw natural pure honey - Beemaid Brand)
1 gram of Nutritional Yeast
I switch back and forth with the above PDYA recipy and the MEA.
And have always gotten great results.
Click here for all the agar recipies that are listed!~
good luck!~
tc
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Laterz, Road
Who the hell you callin crazy?
You wouldn't know what crazy was if Charles Manson was eating froot loops on your front porch!
Brainiac said:
PM the names with  on there names, that means they have mushrooms for sale.
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ThumpaCap
Beer Taster
Registered: 02/19/05
Posts: 568
Loc: Shroomwhere Out There
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Re: Potato Dextrose Agar [Re: Roadkill]
#4312778 - 06/18/05 11:49 PM (6 years, 11 months ago) |
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--------------------
 Look into my heyes !!
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komsija
Stranger
Registered: 03/31/09
Posts: 43
Last seen: 2 years, 11 months
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Re: Potato Dextrose Agar [Re: ThumpaCap]
#10080566 - 03/31/09 01:02 PM (3 years, 1 month ago) |
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i was making agar plate with a potato recipe...PDA
i was doing just the same as was writing here on forums...i clone a oyster mushroom....but i get just a litle colonization after 17 days:(.
I dont now what i am doing wrong:(. after 17 days i see colonization , but its not complitly pure white coler/? Is thet normal, what i am doing wrong?
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Krazy L
Resident Whack Job
Registered: 01/02/09
Posts: 85
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Re: Potato Dextrose Agar [Re: komsija]
#10081266 - 03/31/09 02:45 PM (3 years, 1 month ago) |
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Nice...3 yr old thread. lol
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komsija
Stranger
Registered: 03/31/09
Posts: 43
Last seen: 2 years, 11 months
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Re: Potato Dextrose Agar [Re: Lapius]
#10081280 - 03/31/09 02:47 PM (3 years, 1 month ago) |
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lol...just needed for a help...
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FreeSporePrints
Mycelium Jedi
Registered: 03/06/05
Posts: 1,130
Loc: Rome, ITALY
Last seen: 5 days, 18 hours
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Re: Potato Dextrose Agar [Re: komsija]
#10143557 - 04/10/09 03:16 PM (3 years, 1 month ago) |
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Be sure your pH isn't too much acid or basic.
Be sure your medium into the petri isn't full of water drops.
Be sure your specimen isn't old.
Be sure your oyster mushroom isn't a commercial mushroom. I'm not saying you can't clone commercial specimen, but a lot of time they come from a strain so much expanded that they neither haven't the power to grow a bit.
Try with others oyster first!
Fabio
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fastfred
Old Hand
Registered: 05/17/04
Posts: 6,242
Loc: Dark side of the moon
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Might want to mention that none of these recipies produce PDA they are all modified PDA recipies.
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Chandlerxgore
Stranger
Registered: 08/27/11
Posts: 69
Last seen: 4 months, 9 hours
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Re: Potato Dextrose Agar [Re: fastfred]
#15096813 - 09/18/11 03:15 PM (8 months, 3 days ago) |
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Can you use 1 gram of regular yeast instead of brewer's yeast, yeast extract, or nutritional yeast?
Is there a difference between the yeasts?
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Prisoner#1
Even Dumber ThanAdvertized!
Registered: 01/22/03
Posts: 152,599
Loc: Pvt. Pubfag NutSuck 
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the regular yeast will work but the others are more suited to the task and
typically far cheaper, brewers and nutritional yeast can be found at health
food stores and often in drug stores
BTW... the thread is a couple of years old
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760Rollz
Out the game for now. Peace all
Registered: 03/01/10
Posts: 403
Loc: USA
Last seen: 5 months, 14 days
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Re: Potato Dextrose Agar [Re: Prisoner#1]
#15139382 - 09/26/11 09:25 PM (7 months, 26 days ago) |
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If this thread is that old I might as well ask my question here. I am trying to do agar work. I have made some agar using the recipe RoadKill posted in this thread. The agar that I make only gels up when I put it in the fridge. I don't know where I got the idea that agar should turn to gel, but I'm under the impression you want the agar to gel up. Should it be in a gel state to work with? I can't find any info on it.
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Javadog
Continuing along
Registered: 05/03/10
Posts: 6,009
Loc: USA
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Re: Potato Dextrose Agar [Re: 760Rollz]
#15146221 - 09/28/11 08:15 AM (7 months, 24 days ago) |
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Yes, it should be firm at room temperature.
You may need to add more agar powder.
Good luck,
JD
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Boyd Rice told my brother that life is a corny pack of freesakes
Myco-tek.org
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