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 Gzaajhom
title
Registered: 12/11/10
Posts: 183
Loc: USA
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 Yeast contam on agar? 
#14545396 - 06/01/11 01:50 PM (1 year, 11 months ago) |
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They appeared 2 days after I shot spore solution onto PDA. I used exactly 5 drops around the plate, which then mixed around the whole plate when I hit the table. The pictures are 5 days after inoculation. I thought it might also be bacillus but I'm not sure. The pictures don't really show it, but between the big blobs there are also a bunch of really tiny blobs the size of pin pricks. Also, the picture makes it look more white than it is. The contams have a very noticeable yellow tint. It has a slimy look to it like mucus.
At the time I inoculated to this agar plate, I also did 3 other plates of some other spores, 2 of which are successfully growing mycelium and one which hasn't done anything yet. My sterile technique followed all the rules so I have a feeling the syringe is contammed, but I can't be 100% sure yet until I inoculate another agar plate. If the syringe is contammed and this is yeast I am dealing with, what can I possibly do to get some mycelium? The syringe is 20cc made from a whole spore print (too much yeah).
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Don't hate the player; hate the game. -Abraham Lincoln
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 wood chip
Stranger
Registered: 02/22/09
Posts: 172
Last seen: 1 day, 20 hours
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Re: Yeast contam on agar? [Re: Gzaajhom]
#14555032 - 06/03/11 01:04 PM (1 year, 11 months ago) |
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Pray for a miracle
Yeast grow fast in liquid solutions
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slapphappypill
Enthusiast!
Registered: 11/07/10
Posts: 5,470
Loc: In bed with your mom
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Re: Yeast contam on agar? [Re: wood chip]
#14558883 - 06/04/11 06:53 AM (1 year, 11 months ago) |
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why did you mix the whole plate? NExt time drop the spore drops in place and sector away from contams as the myc grows
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We think we have freedom, but we're all just mice in a maze....
FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!
I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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Gzaajhom
title
Registered: 12/11/10
Posts: 183
Loc: USA
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Quote:
slapphappypill said:
why did you mix the whole plate? NExt time drop the spore drops in place and sector away from contams as the myc grows
I put 5 drops around the plate equal distance from each other. In my clumsiness, they got mixed around. Beside, when people swipe spores on with an inoc loop, they generally hit the whole surface. Anyway, I threw away the plate in the picture
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Don't hate the player; hate the game. -Abraham Lincoln
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