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 Eywa_devotee
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Registered: 10/04/10
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 Eywa's Spores to More Mycelium Cardboard Tek 
#13958694 - 02/14/11 12:55 AM (2 years, 3 months ago) |
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This tek does away with a lot of pitfalls with making a LC or an agar plate from scratch, the only downside is you should use a spore print to make it work instead of a syringe. The spores aren't concentrated enough in most syringes for this tek to work. The main advantages are that it works under less than sterile conditions, and selects for healthy cellulose digesting strains that will almost always get ropey rhisomorphic mycelium that grows great healthy mushrooms.
Materials needed:
Cheap cardboard box, the Chinese toy shipping boxes from walmart work quite well.
Some sort of sugar-water, can use plain sugar, honey or corn syrup
Plastic zip-lock snack bags or pitri dishes
spring or bottled water
Spore print.
Equipment needed:
A microwave oven
Forceps or tweezers that have been sanitized
A syringe or medicine dropper that has been previously sanitized or better yet sterilized
A long needle to scrape the spores and deposit them on the cardboard
lighter to flame the needle with
Coffee cup
The tek:
First nuke the cardboard in a minimal amount of water in the microwave on HI for about a minute. Then while still hot transfer to your plastic bag or pitri dish and cover it. Next put some sugar, corn syrup, or honey in water about 3% solution is ideal by weight/weight and microwave on high in a coffee cup until it boils vigorously. Allow to cool on it's own accord. Next fame sterilize the needle and allow it to cool. Then scrape enough spores from your print until you can see a glob of spores at the end and quickly deposit on the cardboard, make sure you rub them in a little. Next with your sterilized medicine dropper withdraw some of the now cold microwaved sugar water and place a single drop directly on the spot you put the spores on the cardboard. Finally seal the zip lock bag or pitri dish and put in a warm dark place for a week. When you retrieve it a week later, your cardboard should have healthy mycelium on it. From here you can go to either a LC, a grain jar, or culture tube for safekeeping. This technique also works great for cloning mushrooms too! Hope this helps you!
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 Luger0815
noob
Registered: 12/22/10
Posts: 1,677
Loc: @ Home
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 Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: Eywa_devotee]
#13958766 - 02/14/11 01:09 AM (2 years, 3 months ago) |
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I dont get it, what is it good for?
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Eywa_devotee
Stranger
Registered: 10/04/10
Posts: 487
Last seen: 7 months, 5 days
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: Luger0815]
#13958848 - 02/14/11 01:24 AM (2 years, 3 months ago) |
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It is good for starting mycelium from a spore print and auto-selection of good strains.
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Luger0815
noob
Registered: 12/22/10
Posts: 1,677
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: Eywa_devotee]
#13958913 - 02/14/11 01:41 AM (2 years, 3 months ago) |
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And after you grow some myc on your cardboard you have to get some piece's of it to grain.
Or did you plan to throw that cardboard to your "Eywa's Extra Magical Magic Mushroom Substrate!"
So you can skip that "Eywa's Spores to More Mycelium Cardboard Tek" and innoc. the grain direct with spores.
Cardboard is useful if you want to clone a strain under less sterile conditions.
But it is not nearly new.
Im looking forward to your next invention.
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Eywa_devotee
Stranger
Registered: 10/04/10
Posts: 487
Last seen: 7 months, 5 days
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: Luger0815]
#13958983 - 02/14/11 01:58 AM (2 years, 3 months ago) |
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This tek is for germinating spores, not just cloning. It germinates spores, selects good strains, and generates a live tissue culture in one easy step. Spores will not germinate easily on plain cardboard, but they will easily do so with a trace of sugar added to it.
Yes, you can skip the grain step and go straight to a bulk style substrate like the eywa substrate since it selects for strains that can thrive on less than ideal starting substrates high in cellulose. You would just want to use a half quart or so of it to get the spawn started then break it up and start your bulk project.
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado 
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: Eywa_devotee]
#13959838 - 02/14/11 08:47 AM (2 years, 3 months ago) |
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Quote:
Eywa_devotee said:
It is good for starting mycelium from a spore print and auto-selection of good strains.
I'd like to see pics and your control runs, please.
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You make it all possible. Doesn't it feel good?
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k00laid
NEMO
Registered: 05/03/10
Posts: 17,329
Last seen: 2 hours, 49 minutes
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: Eywa_devotee]
#13960885 - 02/14/11 02:00 PM (2 years, 3 months ago) |
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Quote:
Eywa_devotee said:
It germinates spores, selects good strains
care to explain this part in more detail?
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AMU - AMU Q & A - MyVideo Teks!
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virus1824
Mr Mushroom
Registered: 09/25/05
Posts: 1,741
Loc: Europe
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: k00laid]
#13960899 - 02/14/11 02:04 PM (2 years, 3 months ago) |
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So put mycelium cardboard in a LC and hope everything goes well.
Riiight. This is just as good as the semi solid agar LC tek.
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A weekend wasted is never a wasted weekend
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Luger0815
noob
Registered: 12/22/10
Posts: 1,677
Loc: @ Home
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: virus1824]
#13960938 - 02/14/11 02:11 PM (2 years, 3 months ago) |
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No, seriously, you can grow myc on cardboard under less sterile conditions but what will you do with it?????
The cardboard is contaminated, even if the contams doesn't breed on the cardboard.
They will breed at the moment when you get it to Grain, Substrate or whatever.
Cardboard if fun but not useful to skip sterilizing process.
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virus1824
Mr Mushroom
Registered: 09/25/05
Posts: 1,741
Loc: Europe
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: Luger0815]
#13960946 - 02/14/11 02:13 PM (2 years, 3 months ago) |
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yea i know that i have mycelium running  but under less than sterile environments i wouldn't put cardboard in a LC.
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A weekend wasted is never a wasted weekend
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Luger0815
noob
Registered: 12/22/10
Posts: 1,677
Loc: @ Home
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: virus1824]
#13960969 - 02/14/11 02:17 PM (2 years, 3 months ago) |
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Cardboard in LC...unthinkable...hehe
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NeoSporen
Antibiotic cream
Registered: 09/05/09
Posts: 3,957
Loc: Washington State
Last seen: 9 days, 10 hours
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: Luger0815]
#13961100 - 02/14/11 02:46 PM (2 years, 3 months ago) |
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Quote:
Luger0815 said:
No, seriously, you can grow myc on cardboard under less sterile conditions but what will you do with it?????
The cardboard is contaminated, even if the contams doesn't breed on the cardboard.
They will breed at the moment when you get it to Grain, Substrate or whatever.
Cardboard if fun but not useful to skip sterilizing process.
Well put. I was using a tek like this for a while, but kept getting contamination. It's a decent substitute for agar or LC, but hard to get results that are not contaminated. 
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: NeoSporen]
#13961109 - 02/14/11 02:47 PM (2 years, 3 months ago) |
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The idea is to use it with a wild specimen -> cardboard then move myc -> agar for further cleaning.
This thread fails to deliver.
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You make it all possible. Doesn't it feel good?
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ar1es
Psychonaut
Registered: 07/09/10
Posts: 760
Last seen: 5 months, 2 days
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: Doc_T]
#13961266 - 02/14/11 03:24 PM (2 years, 3 months ago) |
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" here is nothing lost or wasted in this life.” - Bhagavad Gita
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jimmyjame1
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Registered: 03/13/10
Posts: 1,926
Loc: 
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Re: Eywa's Spores to More Mycelium Cardboard Tek [Re: Eywa_devotee]
#13962448 - 02/14/11 07:19 PM (2 years, 3 months ago) |
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instead of nuking it could i just boil it in a small pot in shallow water?
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