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 egodeathflux
Guttersnipe
 
Registered: 02/02/10
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 Self-sealing, EASY LC lid TEK.  1
#13621387 - 12/12/10 03:44 PM (2 years, 5 months ago) |
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After some troubles aspirating some thick LC's recently I have made a nice batch of new lids with cannulas/catheters. For me these are free to make, you can order all parts needed cheaply online.
We start with our cannula/catheter.
These badboys attach directly to the end of your syringe (I'm using 20ml as I got a load free), they have a self-sealing bung one end and an open tube the other.
Our assembled syringe+cannula:
Our self-sealing end:
Our open tube end:
They are fitted into lids in the time-honoured silicone+drill fashion.
Our assembled lid:
And finally,
Our fully awesome LC jars:
I don't know if there's similar things out there but these are easy to say the least. They will have tyvek filters added to the other holes when the silicone is dried completely. Can't wait to try them out, results to follow in a week or three. 
UPDATE:
Here, as promised is a pic of a 'something something something fully operational' partially colonised LC jar.
Not the best pic but I have horrible flu and no patience for photography today.
Edited by egodeathflux (12/28/10 01:41 PM)
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Wirenut
Captivity
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 Re: Self-sealing, EASY LC lid TEK. [Re: egodeathflux]
#13621407 - 12/12/10 03:47 PM (2 years, 5 months ago) |
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That's pretty cool 
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k00laid
NEMO
Registered: 05/03/10
Posts: 17,329
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Re: Self-sealing, EASY LC lid TEK. [Re: Wirenut]
#13621467 - 12/12/10 04:00 PM (2 years, 5 months ago) |
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it does. i want to see it work D:
it doesnt sound too easy to me ;p
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AMU - AMU Q & A - MyVideo Teks!
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egodeathflux
Guttersnipe
Registered: 02/02/10
Posts: 2,726
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Re: Self-sealing, EASY LC lid TEK. [Re: k00laid]
#13621722 - 12/12/10 04:48 PM (2 years, 5 months ago) |
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How is it not easy? There is only one part and a syringe.. Plus the parts are medical quality. It's the easiest way I've seen or found. Suggestions or better/easier alternatives are always welcome!
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ProfessorPinHead
Trapped in the Archives....
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Re: Self-sealing, EASY LC lid TEK. [Re: egodeathflux]
#13621868 - 12/12/10 05:10 PM (2 years, 5 months ago) |
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Quote:
egodeathflux said:
How is it not easy? There is only one part and a syringe.. Plus the parts are medical quality. It's the easiest way I've seen or found. Suggestions or better/easier alternatives are always welcome!
 +  +  = 
This one was 9 days old when I took the pic.
I learned how to clone using these lids though.
You can make Lc clones easily w/ them.
It wasn't until later that I upgraded to filter discs.
To make them you just
snip off the tip of 2
3ml syringes, 10 work too,
and glue them into the lid. Then you just
stuff it with poly-fill. You
have to use metal lids unless you rub
the plastic ones with sand paper
first. Otherwize the RTV won't adhere.
 <- 
You can't see it but there is an RTV injection port on the other side right by the little arrow.
The 2 holes add a lot more gas exchange.
I always had problems with 1 hole set ups.
I use 4 holes on my filter disc lids and whole quarts colonize in under 14 days.
I keep them around 78 degrees during colonization.
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"Words hold no power over you until you give in to them".....
myco-tek
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k00laid
NEMO
Registered: 05/03/10
Posts: 17,329
Last seen: 11 hours, 50 minutes
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the availability of the part is the non-easy part i think
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AMU - AMU Q & A - MyVideo Teks!
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 jokefox
Top of the chain
Registered: 12/22/09
Posts: 6,163
Loc: never where I should be
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Re: Self-sealing, EASY LC lid TEK. [Re: k00laid]
#13621988 - 12/12/10 05:38 PM (2 years, 5 months ago) |
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<3
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Morelman
LC Master
Registered: 05/17/06
Posts: 1,010
Loc: Between Right and Wrong
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Re: Self-sealing, EASY LC lid TEK. [Re: egodeathflux]
#13623709 - 12/12/10 11:20 PM (2 years, 5 months ago) |
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Pint LC Jars w/Lids
The silicone I used to glue the turkey injector needle inside the brass tube didn't hold. I switched to gorilla glue ftw.
Waiting on lid gaskets from TMS. 
Not jacking your thread. Just sharing an update.
My signature is my wide mouth lid design from your previous thread....
Edited by Morelman (12/12/10 11:45 PM)
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slapphappypill
Enthusiast!
Registered: 11/07/10
Posts: 5,438
Loc: In bed with your mom
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Re: Self-sealing, EASY LC lid TEK. [Re: Morelman]
#13623912 - 12/12/10 11:52 PM (2 years, 5 months ago) |
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I use 2 holes, one is plugged with silicone glue other has double layer of micropore tape. I use honey LC (4%-10% depending on how much honey comes out) and so far my jars colonize within 8 days and havn't seen any contams yet....
I like you're setup though much more as it looks alot more sterile.  vibes to ya man! let us know how it works, and I forget, did you say what type of LC your using?
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We think we have freedom, but we're all just mice in a maze....
FYI: I stole all my pix off google! F+ PORN! Here is a shit-ton of porn by yours truly!
I have FINALLY written up a couple teks as to how SHP has done things in the past. DISCLAIMER: This is not for the newbie to mycology, and not going to work for everyone! This is simply what works for one person when other teks and methods have failed miserably!
~~~~~How SHP does their unconventional WBS Prep!! (NO DRY METHOD)~~~~
~~~~~SHP's highly disputed method of doing ALL their work outside of a flow hood or a Still air box!~~~~~
~~~~~Troubles harvesting the side and bottom pins in your mono? Learn how!! Dunking included ;-)~~~~~
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Self-sealing, EASY LC lid TEK. [Re: egodeathflux]
#13624675 - 12/13/10 03:10 AM (2 years, 5 months ago) |
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So uh... you haven't PCed one of these yet, have you?
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You make it all possible. Doesn't it feel good?
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egodeathflux
Guttersnipe
Registered: 02/02/10
Posts: 2,726
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Re: Self-sealing, EASY LC lid TEK. [Re: Doc_T]
#13625086 - 12/13/10 08:22 AM (2 years, 5 months ago) |
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Haven't PC's one yet but I know the cannulas are used in autoclaves so that's no concern. Do you foresee a problem?
For my LC's I use LME+Acacia honey and sometimes a bit of gypsum. (Acacia honey is VERY pale so you can see through it well).
The parts are no harder to get than posting on this thread... I know y'all have computers!!! Plus I'm certain there are other people in this community that have access to lab equipment or know someone who does, ya gotta network! Go, make friends with a scientist today, do yourself a favour!
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ProfessorPinHead
Trapped in the Archives....
Registered: 07/09/10
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Re: Self-sealing, EASY LC lid TEK. [Re: egodeathflux]
#13625121 - 12/13/10 08:46 AM (2 years, 5 months ago) |
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"keep it simple stupid" ....
What I mean by that is its not necessary to order supplies to make LC's with when we already have them on hand. 
We put that energy into research instead.
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"Words hold no power over you until you give in to them".....
myco-tek
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sandman420
Baby Daddy
Registered: 06/17/04
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Re: Self-sealing, EASY LC lid TEK. [Re: egodeathflux]
#13625135 - 12/13/10 08:51 AM (2 years, 5 months ago) |
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You know that silicone that you gobbed all around the catheter is self sealing too, right? You could just leave out the catheter completely and seal the hole it is in with silicone and call it a day. Who the hell has a catheter laying around or cares to order some for no reason at all?
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Don't let the bed bugs bite...
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Self-sealing, EASY LC lid TEK. [Re: egodeathflux]
#13625192 - 12/13/10 09:25 AM (2 years, 5 months ago) |
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Quote:
egodeathflux said:
Haven't PC's one yet but I know the cannulas are used in autoclaves so that's no concern. Do you foresee a problem?
No, those are sterilized with ethylene oxide or radiation, not heat.
Not PCable.
Anybody interested in LC lids should take a look at EZ-Port lids: http://www.shroomery.org/forums/showflat.php/Number/10364498
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You make it all possible. Doesn't it feel good?
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Wirenut
Captivity
Registered: 09/14/10
Posts: 416
Loc: Gulf Coast
Last seen: 1 year, 1 month
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Re: Self-sealing, EASY LC lid TEK. [Re: Doc_T]
#13625234 - 12/13/10 09:49 AM (2 years, 5 months ago) |
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Silicone catheters are autoclavable.. You're fine.
You have access to this stuff and built something out of it..
So the idea can lead people to create something similar with stuff they do have access to..
I'm interested to see how it works.
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Self-sealing, EASY LC lid TEK. [Re: Wirenut]
#13625261 - 12/13/10 10:02 AM (2 years, 5 months ago) |
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Well we can talk about it, or RP can toss one in the PC.
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You make it all possible. Doesn't it feel good?
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jmello
Mycosapien
Registered: 01/27/08
Posts: 263
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Re: Self-sealing, EASY LC lid TEK. [Re: Doc_T]
#13625284 - 12/13/10 10:13 AM (2 years, 5 months ago) |
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I've thought about doing this cuz i have access to medical supplies (legally) but I wondered if after sucking up some myc that the myc left in the tube would grow and block the cath?? I dunno tho, clean work man looks good!
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"Just can't live that negative way..."~Bob Marley
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Doc_T
Random Dude
Registered: 03/06/09
Posts: 42,395
Loc: Colorado
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Re: Self-sealing, EASY LC lid TEK. [Re: jmello]
#13625293 - 12/13/10 10:16 AM (2 years, 5 months ago) |
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There's a better way to do it. Click my link upthread.
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You make it all possible. Doesn't it feel good?
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ProfessorPinHead
Trapped in the Archives....
Registered: 07/09/10
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Re: Self-sealing, EASY LC lid TEK. [Re: Doc_T]
#13625303 - 12/13/10 10:18 AM (2 years, 5 months ago) |
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"Words hold no power over you until you give in to them".....
myco-tek
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Base Icks
Registered: 03/19/10
Posts: 5,905
Loc: Shroomshire
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My lids are silicone IP, and silicone around a peice of filter disk for GE. I use a 16g needle on that and call it a day.
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