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 whiterasta
Day careobserver
Registered: 04/09/02
Posts: 1,780
Loc: Oregon
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 Culture of C.paspali for alkaloidal production, No Acid talk please! 
#7018848 - 06/07/07 09:51 AM (4 years, 11 months ago) |
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Since it has been demonstrated that the ergoline profile of Claviceps paspali is very similar to MG or HBWR and has produced up to 1mg/ml in LC. These are my thoughts on strain isolation and culture for alkaloidal extraction.
A simple search will locate not only raw sclerotia,but also culture slants of C.paspali.
The raw sclerotia seems like the best bet for isolating a potent strain than chancing an ag library to have a high alkaloid strain. So basic wild print isolation techniques on agar would begin your quest for a "potent" strain. The difference with the fungi here is with a black light one can determine the alkaloid content roughly by how much it glows. So isolation sectioning should be selective for the brightest flourescing strains. Once one has a sterile strain selected for high alkaloid content it is used to inoculate a Liquid Culture using media already targeted to produce the most desired alkaloids
media for LC media maximizing Lysergic acid hydroxyethyamide production in C.paspali
Production of Simple Lysergic Acid
Derivatives and Paspalic Acid
Lysergic acid is produced by the strain Claviceps paspali. It.
synthesizes (+)-lysergic acid-a--hydroxy-ethylamide in sub-
merged culture. This substance can be converted into
(+)-lysergic acid amide, and on alkaline hydrolysis results into
(+)-lysergic acid. Lysergic acid is a key substance, in the production of ergot alkaloids and can be employed in, the chemical synthesis of their derivatives. Paspalic acid functions as the precursor of (+)-lysergic acid.
The liquid culture medium
Chemical Amount
Sorbitol/50 g
Succinic acid/36g
KH2PO4/2g
MgSO4.7H2O/0.3g
NaOH to PH 5.4
FeSO4.7H2O/10mg
ZnSO4.7H2O/1mg
Water to 1ltr.
Culture in and keep in low watt red light (40w is enough)
Alkaloid concentrations of 1mg/ml have been reported with agitated or aerated LC cultures.
Extraction of alkaloids could be simply be by adding a mild base to the culture and extracting with a suitible solvent ( remember red lights only! and as little air to the extract as possible) then salting out with tartaric acid. A rotovape would make the final drying of the extract quick and airless but kept dark and with good ventilation even at half the stated concentrations and only getting a 50% yield on the extraction. 250mg of faily pure mixed LSA's should be available per liter with the profile loaded to the more psychedelic hydroxyethylamide.
This is theory based on patent searches and research docs and I have not done this myself. I put it out as info for those who may pursue this avenue which while I am not able to pursue ,I believe hold some very important avenues for biological creation of a very special molecule.
I am VERY rusty but acetaldehyde and ethanol in a mild tartaric acid solution ( wine) could perform something very cool with the paspalic ergolines perhaps
WR
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To old for this place
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 dirtworshipper
Sitting in theheart cave
Registered: 05/12/07
Posts: 2,054
Loc: at The Guru's lotus feet
Last seen: 4 days, 8 hours
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 Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: whiterasta]
#7019032 - 06/07/07 10:52 AM (4 years, 11 months ago) |
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oh wow
thanks
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“You've got as many lives as you like, and more, even ones you don't want.” - George Harrison
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Entersandman
Wings of Tomorrow
Registered: 06/19/06
Posts: 521
Loc: behind the border
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Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: dirtworshipper]
#7019563 - 06/07/07 01:36 PM (4 years, 11 months ago) |
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thank you very much for this very useful information.
did you obtain a culture of Paspali??
peace
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LightShedder
Staring at Rhizos
Registered: 08/30/05
Posts: 2,833
Loc: Amazonia
Last seen: 1 month, 22 days
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Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: Entersandman]
#8850918 - 08/30/08 01:15 AM (3 years, 8 months ago) |
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I hope nobody minds me bumping this but I was very curious about this topic and thought that it'd be better to ask a question here rather than start a new topic. When you mention "basic wild print isolation" are you talking about good ole fashioned agar like the type used for various mushroom isolations? And then looking for the most florescent growth on that agar? I've always wondered about ergot strain isolation and this seems pretty relevant.
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One day, God created LSD through the intercession of blessed Albert Hoffman. This powerful sacrament then went on to enlighten mankind, even the president of america. This ushered in an age of spiritual unity and peace. This allowed for mankind to reach it's full potential including immortality and intergalactic exploration and habitation. Thanks, God.
"If it seems radical, pretend like it is normal."
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mbrown3391
Stranger
Registered: 08/22/08
Posts: 137
Last seen: 1 year, 9 months
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Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: LightShedder]
#8851375 - 08/30/08 07:28 AM (3 years, 8 months ago) |
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Can you provide a link for such a website? i cannot find a single site that would sell Claviceps paspali samples.
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shroomedalice
self titled
Registered: 11/23/09
Posts: 103
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Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: mbrown3391]
#13057666 - 08/15/10 11:35 PM (1 year, 9 months ago) |
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I thought you said no acid talk.
and in the same post your trying to tell us how to do a hydrolisis
on lysergamides and how to isomerise paspalic acid too lysergic acid.
then you just drop the subject wow man what do you want
some BEE to just drop in and hand it too you.
but if anyone is realy interested in talking about culturing this
wonder of medicine then I am very interested.
to get the ball rolling as some of us do more than just copy some
shit of rhodiums archive 
this is a pic from some of the russian BEE's doing a liquid culture
also I have attached for the ozzies here a paper on claviceps paspali
in australia. it is quite detailed and will show you were to
find this wonder.
for those who know were to find me there is a lot more info there.
BEE safe thanx for the info on the shrooms you guys rock.
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shroomedalice
self titled
Registered: 11/23/09
Posts: 103
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 Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: shroomedalice]
#13057765 - 08/16/10 12:13 AM (1 year, 9 months ago) |
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Log in to view attachment
and to keep the ball rolling ive attached a paper on doping the ergot
with tryptophan.
this will work for paspali as well as purpurea as its the building
block for the lysergic part of the molecule not the amide function.
you will notice that unlike shrooms ergots build off of amino acids.
were as shrooms build off of tryptamines.
I belive that if we add substitutions to the tryptophan we will get
substituted paspalic acid much like using 5-methoxy-tryptamine will
give you 5-methoxy-4-hydroxy-dimethyltryptamine when growing shrooms
Edited by shroomedalice (08/16/10 12:22 AM)
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Sgt. Frost
Registered: 04/16/10
Posts: 254
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Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: shroomedalice]
#13058520 - 08/16/10 08:54 AM (1 year, 9 months ago) |
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This thread has long been dead, Shroomedalice. No one has commented on it for over a year, and even then, they should have not commented on it.
It's too old and you can certainly find newer information in a newer thread.
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FilamentousFungi
Entheomycologist
Registered: 08/23/10
Posts: 487
Loc: Down the rabbit hole!
Last seen: 6 months, 13 days
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Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: Sgt. Frost]
#13098244 - 08/24/10 11:04 PM (1 year, 8 months ago) |
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Look for the acrticles by Amicci '69, and Mantle '76. Those two should get you what you're looking for. There has been a lot of talk of adding amines in the fermentation broth recently. People are saying that they have been successful in getting natural LSD from that method. The uptake and use of said amides by the fungus is what is interesting. I believe that there are articles about basic amide uptake that give specific broth recipes. The additives and concentrations of atmospheric gasses are the key to getting fungi to uptake the amides. The strain of claviceps detailed by Amicci & Mantle would be the strain to use if I were to guess due to it's high yield of specific amide(ergotamine).
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Edited by FilamentousFungi (08/24/10 11:14 PM)
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FilamentousFungi
Entheomycologist
Registered: 08/23/10
Posts: 487
Loc: Down the rabbit hole!
Last seen: 6 months, 13 days
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Re: Culture of C.paspali for alkaloidal production, No Acid talk please! [Re: shroomedalice]
#13098273 - 08/24/10 11:18 PM (1 year, 8 months ago) |
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Quote:
shroomedalice said:[/b
you will notice that unlike shrooms ergots build off of amino acids.
were as shrooms build off of tryptamines.
There have been articles stating that tryptamines are used along amino acids. The strain of ergot, and the relative clavines it produces, are what decides how much amino acids/tryptamines are used.
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