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wisp
Registered: 04/13/08
Posts: 5,304
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. *DELETED* *DELETED*
#12707406 - 06/08/10 04:37 AM (1 year, 11 months ago) |
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Post deleted by wispReason for deletion: .
Edited by wisp (09/08/11 07:10 PM)
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Mephistophelian
Quasi Hob-Nobbery


 Registered: 08/14/08
Posts: 2,503
Loc: Camp Crystal Lake
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Re: Fruiting Cordyceps militaris off plant-based substrates. [Re: wisp]
#12707485 - 06/08/10 05:36 AM (1 year, 11 months ago) |
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There is a bank of links from Speeker, not just a couple. Its a whole page of links, it has the recipe for the cordycep substrate that Wbatz used that contains no insects in it at all.
If I find the link I'll post it, but I wanted to verify for you that you are indeed correct. I remember printing it out and finding it interesting that there was no need for insects or insect powder.
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wisp
Registered: 04/13/08
Posts: 5,304
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Re: Fruiting Cordyceps militaris off plant-based substrates. [Re: Mephistophelian]
#12707578 - 06/08/10 06:28 AM (1 year, 11 months ago) |
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Ah, brilliant, mentioning that wbatz tried it helped. I had a look at Speeker's links, but was overwhelmed.
I remember that thread now. It was incredible!
He linked to this article. I'm not sure if that was actually the one, but it doesn't appear to use insects at all.
To quote from wbatz's thread, of the method he used:
Quote:
This is a summary of the recipe and the methodology used on my first experience with Cordyceps:
Cook peeled and sliced potatoes in mineral water or distilled water on low heat until tender. Filter the broth through a sieve and discard the potatoes.
Potato Broth
500 g potatoes (peeled and sliced thin) 5000 ml water (distilled or mineral water)
Prepare the following nutrient solution using the potato broth prepared above:
Nutrient Solution
200 ml potato broth 2 g KH2PO4 1 g MgSO4 1 g C6H14N2O7 (Ammonium citrate) 30 g glucose (dextrose) 3 g Soybean peptone 50 mg vitamin B1 (thiamine)
Dilute all the above components with water by adjusting the volume to 1000 ml
Measure 35 ml of nutrient solution and pour into wide mouth glass jars. Weigh the rice grains (30g) and put them in the jars along with the nutrient solution. Close the glass flask with a metal lid with a felted disc under the lid. You do not need a hole in the metal lid, only the filter under the lid is enough for the exchange of air during the growth of the mycelium.
Per jar :
35 ml nutrient solution 30 g rice (polished or brown rice)
After sterilization, inject approximately 5 ml of the Liquid culture of Cordyceps militaris into every glass jar (use a micropipette with sterilized tips or even a stainless spoon sterilized on the flame of a Bunsen burner). Incubate in the dark (25ºC) until the mycelium cover all the grains.
Primordia initiation
Start a one week treatment for the initiation of primordia by putting the flasks on the refrigerator at night and leaving them at an illuminated area during the day (18-25 ºC), without direct sunlight. After the appearance of primordia , keep the flasks in a 18-23ºC environment with indirect light until the mushrooms develop to full size.
You can find more details about the environmental conditions on the following translated link (indicated by Speeker) :
http://translate.google.com/translate?hl=en&sl=zh-CN&u=http://www.ngx.net.cn/yuanjiaomeiti/dianshi/jiemujieshao/t20090428_22870.htm
Does anyone still have Roger Fudd's C. miliaris culture available for trade? I should have jumped at the opportunity when I had it.
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speedy
Stranger


Registered: 08/15/07
Posts: 73
Loc: Nth Victoria, Australia
Last seen: 17 hours, 1 minute
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Re: Fruiting Cordyceps militaris off plant-based substrates. [Re: wisp]
#12712589 - 06/08/10 10:10 PM (1 year, 11 months ago) |
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Thanks guys, I'll have to try this with C.hawksii. I got some pieces onto agar a couple days ago and i's just showing signs of growth now. Looking good so far.
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RogerRabbit
Bans for Pleasure


Registered: 03/26/03
Posts: 36,969
Loc: USA Mountain Northwest
Last seen: 5 hours, 32 minutes
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Re: Fruiting Cordyceps militaris off plant-based substrates. [Re: wisp]
#12714024 - 06/09/10 07:51 AM (1 year, 11 months ago) |
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Quote:
tripsis said: Some time ago, I think I recall Speeker posting a link about a method invented in China for fruiting Cordyceps from rice. Unfortunately, I can't find the link.
I saved it. http://translate.google.com/translate?hl=en&sl=zh-CN&u=http://www.ngx.net.cn/yuanjiaomeiti/dianshi/jiemujieshao/t20090428_22870.htm
Unfortunately, it shows the limits of Google translate. RR
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