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 x7x_x7x
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 resistance to contams 
#12667560 - 06/01/10 06:39 PM (2 years, 11 months ago) |
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i'm wonderimg why some cultures become contaminated after some time in frigde. that was happen a couple of years ago with golden teacher and now with Z. a slight green mold appear in the agar plate after one month or so. could be a genetic problem of the isolate?
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 Sgt. Frost
Registered: 04/16/10
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 Re: resistance to contams [Re: x7x_x7x]
#12667587 - 06/01/10 06:44 PM (2 years, 11 months ago) |
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Doubt it. I think it must have landed there soon before you placed the plate in the fridge or it had gotten into the plate from the outside. Or it had always been there but too small of a colony to see then multiplied after being put in the fridge. Don't know 
Interested in other responses.
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Sci-Fi
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Re: resistance to contams [Re: x7x_x7x]
#12667778 - 06/01/10 07:15 PM (2 years, 11 months ago) |
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Did the green start growing on un-colonized agar or on the existing myc culture?
Was the plate fully colonized?
Was the plate sealed with glad wrap or parafilm before it was put into cold storage?
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x7x_x7x
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Re: resistance to contams [Re: Sci-Fi]
#12667820 - 06/01/10 07:24 PM (2 years, 11 months ago) |
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the plate was full colonized when the green appears, and was hermetically sealed.
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Sci-Fi
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Re: resistance to contams [Re: x7x_x7x]
#12667855 - 06/01/10 07:32 PM (2 years, 11 months ago) |
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Sounds like bluing, which actually ranges in color from blue to green.
I have seen some cubes blue almost totally green after being picked
All my plates blue after being in the fridge for a month.
It's also unlikely a contam will start growing on live myc.
Is it possible this isn't contaminant mold?
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x7x_x7x
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Re: resistance to contams [Re: Sci-Fi]
#12667989 - 06/01/10 07:54 PM (2 years, 11 months ago) |
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i have grown aparenlty clean sections of these cultures in LC and results 100% contaminated
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Isolatedwedge
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Re: resistance to contams [Re: x7x_x7x]
#12668091 - 06/01/10 08:10 PM (2 years, 11 months ago) |
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believe it or not there are pores in petri dishes. how many times have you used the dish?
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x7x_x7x
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never used. the green appears spontaneously. i think the green is since the day of inoculation. but, why happen only in certain isolates?
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TexasMyco
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Re: resistance to contams [Re: x7x_x7x]
#12668894 - 06/01/10 10:30 PM (2 years, 11 months ago) |
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since it was never used, im going to assume it was already present and those isolates had genetics making them more prone to contam.
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RogerRabbit
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Re: resistance to contams [Re: TexasMyco]
#12669059 - 06/01/10 11:01 PM (2 years, 11 months ago) |
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Some types of mycelium can anamorph into others. For example cordyceps have many known anamorphs. I've not heard of this with cubensis, but we've all seen otherwise good mycelium go bad, so don't rule it out. There's an awful lot we don't know about mushrooms.
RR
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fastfred
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It's just a contaminated plate finally displaying its self. I've seen the same thing many times. It is often that one plate contams and eventually it spreads.
Rest assured that "spontaneous generation" is still known to be an invalid theory.
-FF
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CheeWiz
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Re: resistance to contams [Re: fastfred]
#12681679 - 06/03/10 11:59 PM (2 years, 11 months ago) |
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Hi; I agree with Fastfred. The once healthy mycelium has become weakened over time allowing a competitor that has been held in check to take over. Many times a competitive fungi mycelium can look very much like the mycelium of the fungi you’re trying to culture till it starts to sporulate. Then you will see the color of the spores (blue, green, brown, black, ex.) .
The way I can test for this is by preparing a microscope slide that I've albumen in the center of for the sample to stick to, a drop of DI water, a cover slip and a microscope. FF would be better at explaining how prepare an albumened slide or an equivalent than myself. What you want to do is get a loop of the growth in question place it on the slide, add a drop of water and then the cover slip. You're good to look at it under the microscope now. If it's spores you should be able to see them and the same is true of the mycelium bluing.
Edited by CheeWiz (06/04/10 12:08 AM)
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PrimalSoup
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Quote:
believe it or not there are pores in petri dishes.
Not.
Quote:
the green appears spontaneously. i think the green is since the day of inoculation. but, why happen only in certain isolates?
I've had apparently healthy cultures that carried contams, the contams would only show up under certain environmental conditions - although the one you've described is kind of strange... Mushrooms have parasites and the parasites have highly evolved systems for survival and colonization.
"I say we take off and nuke the entire site from orbit. It's the only way to be sure."
Peace
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iluvfungi
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Re: resistance to contams [Re: PrimalSoup]
#12682033 - 06/04/10 01:13 AM (2 years, 11 months ago) |
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Check this one out. I have some pyrex petri dishes. I poured agar sloppy (hey it was the first time), so a little is on the outside of the dishes. The dishes are wrapped with parafilm. You can literally see trich on the bottom of the plate, outside the culture where small amounts of agar accumulated. Yet, the inside culture has no trich (been months and no trich is inside still).
My point is, if you sealed the plate firmly with parafilm; ain't nothing getting into it. So it must have already been their in the first place. We are talking about microscopic (invisible) spores here for bacteria.
On the bright side, you'd think after exposure to bacteria time and time again, fungi would learn to eat the bacteria. I'm keeping all my trich infected plates and going for it.
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PrimalSoup
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Re: resistance to contams [Re: iluvfungi]
#12685258 - 06/04/10 03:52 PM (2 years, 11 months ago) |
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They do eat the bacteria... It's fun to watch, too!
BTW I've had agar plates in a sealed container remain contam free for over 10 years.
Peace
-PS
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