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 NoFaceToSave
Musician
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 What to do about senescence 
#10119485 - 04/06/09 07:42 PM (3 years, 1 month ago) |
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If i harvested spores off the fruits would they cultivate with the original vigor? What about tissue from the base of a fruit body?
Id really like to keep this strain going if its at all possible
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“By calling attention to ‘a well regulated militia’, the ’security’ of the nation, and the right of each citizen ‘to keep and bear arms’, our founding fathers recognized the essentially civilian nature of our economy. Although it is extremely unlikely that the fears of governmental tyranny which gave rise to the Second Amendment will ever be a major danger to our nation, the Amendment still remains an important declaration of our basic civilian-military relationships, in which every citizen must be ready to participate in the defense of his country. For that reason, I believe the Second Amendment will always be important.”
John F. Kennedy, April 1960
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neopet nub
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Spores would be like a new MS grow. (no senescence)
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Ego death from weed!
Edited by neopet nub (04/07/09 05:23 AM)
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Buckeye Oysters
From Zero to Hero
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Re: What to do about senescence [Re: neopet nub]
#10120026 - 04/06/09 09:06 PM (3 years, 1 month ago) |
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Quote:
A tissue would have to be cloned and would not be effected by senescence either.
If you clone from a fruit you will preserve the age of the mycellium and yes senescense will be a factor.
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Evolution is Lamarckism in disguise. Adaptation never creates a new species or trait, but rather the new species/trait always existed within the parent DNA until circumstances allowed it to be activated. For instance, every wolf has the DNA for poodles, but that DNA would never be revealed without man selectively breeding for it.
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NoFaceToSave
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So collecting spores is the way to go?
I'll steralize some empty jars with notecards in them, Take some good looking caps out of the monotub and and place them in the jars inside a disenfected glovebox.
Then i'll have to formulate some AGAR to isolate good ropey mic.
Sounds bout right?
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“By calling attention to ‘a well regulated militia’, the ’security’ of the nation, and the right of each citizen ‘to keep and bear arms’, our founding fathers recognized the essentially civilian nature of our economy. Although it is extremely unlikely that the fears of governmental tyranny which gave rise to the Second Amendment will ever be a major danger to our nation, the Amendment still remains an important declaration of our basic civilian-military relationships, in which every citizen must be ready to participate in the defense of his country. For that reason, I believe the Second Amendment will always be important.”
John F. Kennedy, April 1960
Edited by NoFaceToSave (04/06/09 10:27 PM)
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Frequency
Elemental
Registered: 03/25/09
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Quote:
NoFaceToSave said:
So collecting spores is the way to go?
Absolutely, however there's nothing wrong with giving the ol' college try when using tissue to inoculate. Senescence = age. The older your fruit body tissue, the worse the chance becomes at successful inoculation and contamination prevention.
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McKenna: I think that I am always, from the very first psychedelic experience, had the uncanny intuition that "Yes this has been around for 50,000 years" but it somehow going to be critically important in our lifetime. That we will need it for something. Maybe just to think our way out of the mess that we're getting into.
Shulgin: If it's needed, we have it. That's the beauty. The tool is there. For the time when the need is obvious. And this may be our lifetime...
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 The shroomy 1
Luminous beings surround me
Registered: 03/27/07
Posts: 5,054
Loc: Texas
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Re: What to do about senescence [Re: Frequency]
#10120561 - 04/06/09 10:38 PM (3 years, 1 month ago) |
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Quote:
If i harvested spores off the fruits would they cultivate with the original vigor?
YES
Quote:
What about tissue from the base of a fruit body?
That Would Be Cloning.
Quote:
Id really like to keep this strain going if its at all possible
Making prints from the Fruitbodies is the simple solution.
Senescence will not be an issue until you have cloned many many times off the original fruit. I seriously don't think you should be worried about such things at this point in your cultivation experience.
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NoFaceToSave
Musician
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Quote:
The shroomy 1 said:
Quote:
If i harvested spores off the fruits would they cultivate with the original vigor?
YES
Quote:
What about tissue from the base of a fruit body?
That Would Be Cloning.
Quote:
Id really like to keep this strain going if its at all possible
Making prints from the Fruitbodies is the simple solution.
Senescence will not be an issue until you have cloned many many times off the original fruit. I seriously don't think you should be worried about such things at this point in your cultivation experience.
I've been keeping this strain going with grain 2 grain for over a year (just about a year exactly), it seems to be tuckered out. I'll take the jars i froze, start tubs and try to collect spores from the fruits. That should fix everything it sounds like!
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“By calling attention to ‘a well regulated militia’, the ’security’ of the nation, and the right of each citizen ‘to keep and bear arms’, our founding fathers recognized the essentially civilian nature of our economy. Although it is extremely unlikely that the fears of governmental tyranny which gave rise to the Second Amendment will ever be a major danger to our nation, the Amendment still remains an important declaration of our basic civilian-military relationships, in which every citizen must be ready to participate in the defense of his country. For that reason, I believe the Second Amendment will always be important.”
John F. Kennedy, April 1960
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RogerRabbit
Bans for Pleasure
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When you go back to spores you lose the strain. Remember, a strain is a pairing of compatible hyphae. The way to avoid senescence is to make master culture slants from juvenile cell lines of the strain you wish to preserve.
RR
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www.mushroomvideos.com
semper in excretia sumus solim profundum variat
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NoFaceToSave
Musician
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Re: What to do about senescence [Re: RogerRabbit]
#10163217 - 04/14/09 09:10 AM (3 years, 1 month ago) |
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Thanks RR, aparently i have a ton of research to do on these terms
"master culture slants"
"Juvenile cell lines"
I might wan't to latch onto a microbology book from the library and become real farmilier with it.
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“By calling attention to ‘a well regulated militia’, the ’security’ of the nation, and the right of each citizen ‘to keep and bear arms’, our founding fathers recognized the essentially civilian nature of our economy. Although it is extremely unlikely that the fears of governmental tyranny which gave rise to the Second Amendment will ever be a major danger to our nation, the Amendment still remains an important declaration of our basic civilian-military relationships, in which every citizen must be ready to participate in the defense of his country. For that reason, I believe the Second Amendment will always be important.”
John F. Kennedy, April 1960
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veda_sticks
Cultivator
Registered: 07/29/07
Posts: 11,658
Last seen: 1 month, 18 hours
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Quote:
NoFaceToSave said:
Quote:
The shroomy 1 said:
Quote:
If i harvested spores off the fruits would they cultivate with the original vigor?
YES
Quote:
What about tissue from the base of a fruit body?
That Would Be Cloning.
Quote:
Id really like to keep this strain going if its at all possible
Making prints from the Fruitbodies is the simple solution.
Senescence will not be an issue until you have cloned many many times off the original fruit. I seriously don't think you should be worried about such things at this point in your cultivation experience.
wow thats along time!!!
I've been keeping this strain going with grain 2 grain for over a year (just about a year exactly), it seems to be tuckered out. I'll take the jars i froze, start tubs and try to collect spores from the fruits. That should fix everything it sounds like!
dman thats along time to be doing g2g
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Roger Rabbit and Road Kill cultivation videos Starting out-This site will answer a lot of questions, great pf tek video guide
Agar's Grain LC Tek
Lipas Deli cup Agar Tek
Cakes suck for pins - click me do it right
Spice - 
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neopet nub
Stranger
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Re: What to do about senescence [Re: veda_sticks]
#10172097 - 04/15/09 01:59 PM (3 years, 1 month ago) |
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He might have amazingly low temperatures lol.
I have one friend whos house is so cold that it took 1.5 months to colonize a 1/2 pint.
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Ego death from weed!
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