| BlimeyGrimey |
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(Doctor of FoolyC |
| 05/15/08 06:47 PM |
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Re: Tissue culture (moved)
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Quote:
solumvita said:
Hi Mak
Not sure if you want help on tissue culture or cloning. Mostly cloning involves taking a clean (generally use the inside of a mushroom near to where it forms the spores, as this is the most genetically diverse part), piece of tissue from a mushroom that is already growing and then allowing it to grow on agar medium.
When cloning you usually DON'T want genetic diversity. A clone usually is a exact copy of the genetics of the mushroom being used. Any part of the mushroom would work for cloning since all the tissue that makes up the mushroom is mycelium in a different form. Any piece will go back into vegetative form once placed on agar.
If you want genetic diversity start from spores.