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Mushrooms, Mycology and Psychedelics >> Mushroom Cultivation >> Mushroom Cultivation Archive >> Panaeolus
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maxphun
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Reged: 09/25/02
Posts: 32
Panaeolus Cyanescens (Wild print - Florida)**UpdateFeb25No.2
      02/13/03 10:07 PM

Species: Panaeolus cyanescens / Copelandia cyanescens
Strain: Florida (wild) Manatee County

Agar Culture

Multi-spore onto agar (Light malt & yeast)
Isolated healthy/fast growing section.
Transferred to strong peroxide agar.

Incubation Temp: 75 ? 85 F



Grain Culture

Wheat grain was simmered for 45 min, drained, then pressure-cooked for 1hour 30 min.
Inoculated 6 x 1 pint Jars with Wheat Grain with 3 agar wedges each inside a homemade glove box.



1 jar out of six stalled no visual sign of contamination but a strong coco smell.

Full colonisation took 2 weeks.

Incubation Temp: 70 ? 85 F



Substrate Preparation

2 Jars Used to spawn pasteurised Hay (Oven pasteurised 170 F for 2 hours).

Hay was submerged in water for 48 hours before pasteurising.



Spawning & Incubation

Hay was mixed with colonised wheat grain, covered with plastic (wholes punched).

Incubation Temp: 70 ? 78 F

One week later Hay is nearly colonised.




Spawning & Casing Failures

1 Jar was cased in the jar with composed horse dung (***Contaminated***)
2 Jars where used to spawn serialised composed dung and straw. (**little growth **stalled **)

Casing

2 parts Peat and Loam mix
2 parts Vermiculite
1 part fine Limestone grit
2 parts Water

Oven pasteurised 170 F for 2 hours

Casing layer applied.

Depth: 2/3 Inch



Casing layer colonising



Incubation Temp: 70 ? 75 F (casing surface only) but I would guess the temperature was significantly higher below casing layer.

Primordia conditions have now been introduced....

Primordia Formation Procedure

Humidity: Supplied from wet perlite.
Air temp: 70 - 75 F (from 20th Feb temp = 75 - 84 F)
Air Exchange: 1.5 per hour from aquarium bubbler 18/24
Air Circulation: Computer Fan running 18/24
Light: Diffuse natural light
Exhaust: 4 holes tightly filled with synthetic pillow stuffing

First Pins 3 days after starting the primordia formation conditions.



//Update Feb 25th//

Cropping

Humidity: Supplied from wet perlite. Very High due to bottom heat.
Air temp: 75 - 84 F
Air Exchange: 1.5 per hour from aquarium bubbler 24 hour
Air Circulation: Computer Fan running 24 hour
Light: Diffuse natural light
Exhaust: 4 holes tightly filled with synthetic pillow stuffing



//end update//

Credits

Shroomery Forums (Thanks :smile:)
Stamets & Chilton (1883)The Mushroom Cultivator.

Questions

Does the hay look ready to case? **ANSWERED**
Does the casing Layer sound good? Concerns over burning mycelium with the lime **ANSWERED**
What was the strong coco smell (contamination???)?

Note

This is my first attempt at mushroom cultivation.
I will keep you all updated.

--------------------


Edited by maxphun (02/25/03 05:27 AM)

Post Extras Print Post   Remind Me!     Notify Moderator
. * * Re: Panaeolus Cyanescens (Wild print - Florida)**UpdateFeb25No.2 GGreatOne234   04/18/05 04:30 PM
. * * Re: Panaeolus Cyanescens (Wild print - Florida)**Update Feb 24** maxphun   02/23/03 09:18 PM
. * * Re: Panaeolus Cyanescens (wild print:Florida) maxphun   02/22/03 09:12 AM
. * * Re: Panaeolus Cyanescens (wild print:Florida) mix_up   02/22/03 09:58 PM
. * * Re: Panaeolus Cyanescens (wild print:Florida) maxphun   02/22/03 11:05 PM
. * * Re: Panaeolus Cyanescens (wild print:Florida) psyphon   02/23/03 07:48 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) MisterZ   02/14/03 01:50 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/14/03 10:23 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) mix_up   02/15/03 03:32 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/15/03 06:04 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/15/03 09:56 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) AnnoA   02/15/03 11:16 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/17/03 12:48 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/18/03 01:35 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) Raadt   02/18/03 08:38 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) MisterZ   02/19/03 07:47 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) BlueJay   02/19/03 10:01 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/19/03 11:17 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/20/03 04:23 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) Raadt   02/20/03 04:26 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/20/03 04:34 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) Raadt   02/20/03 07:20 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/19/03 09:23 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/24/03 08:59 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/25/03 05:25 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) BlueJay   02/25/03 10:17 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/26/03 10:31 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) BlueJay   02/26/03 03:41 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/26/03 09:37 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/26/03 10:04 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) mix_up   02/27/03 07:00 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) BlueJay   02/28/03 05:32 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) Raadt   02/28/03 10:28 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) MisterZ   02/28/03 02:42 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) BlueJay   02/28/03 05:31 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) Raadt   02/27/03 04:23 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) MisterZ   02/25/03 10:27 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/25/03 10:58 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/25/03 11:24 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) mix_up   02/25/03 09:49 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) Raadt   02/19/03 12:47 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) blahblahblah   02/19/03 05:38 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) BlueJay   02/19/03 08:15 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) mix_up   02/16/03 06:16 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) comario2   02/14/03 01:36 PM
. * * Re: Panaeolus Cyanescens (wild print from Florida) Joshua   02/14/03 06:17 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) AnnoA   02/14/03 12:08 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) maxphun   02/14/03 12:33 AM
. * * Re: Panaeolus Cyanescens (wild print from Florida) angryshroom   02/14/03 04:54 AM


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