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 Holydiver
Registered: 03/19/01
Posts: 4,986
Loc: The midnight sea
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 Trypticase Soy Broth - uses?
#8291922 - 04/16/08 03:35 PM (5 months, 22 days ago) |
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They were throwing out a case of this stuff at work because it "expires" in a week, so I acquired it. Still sealed and sterile in 100ML bottles. Any particular uses here? Anyone experimented adding it to agar plates or LC mixes?
"Trypticase Soy Broth (TSB) is a nutritious medium that will support the growth of a wide variety of microorganisms, including common aerobic, facultative and anaerobic bacteria and fungi.1-4 Because of its capacity for growth promotion, this formulation is included in the USP as a sterility test medium.5
TSB is also recommended, because of growth promotion, for use as the inoculum broth for disc diffusion and agar-dilution antimicrobial susceptibility testing as standardized by the Clinical and Laboratory Standards Institute (formerly NCCLS)."
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To find a place to live between the negatives and positives.
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 MycoAu
Registered: 07/18/07
Posts: 475
Last seen: 10 hours, 12 minutes
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 Re: Trypticase Soy Broth - uses? [Re: Holydiver]
#8295893 - 04/17/08 11:56 AM (5 months, 22 days ago) |
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I have some of the same stuff. Also from work- though it wasn't "acquired". Anyway, the only thing I've tried to use it for was the possibility of germinating Lentinula edodes spores. Failed. Of course, I also failed to germinate them on agar also. It took a pre-hydrated (24 hour soak in sterile water) before a multi-spore inoculation into a sterilized jar of WBS to germinate them.
I plan on trying some LC stuff with the TSB once I get a few of my cultures cleaned up (new species for me).
If you're going to use the stuff, I'd dilute it with sterile water to 1/2 strength or less and then see how inoculating with a peice of agar works for you. (Then try working up to higher strengths to see if you can increase speed and health of the colony.)
If nothing else, use it for soaking your grain to increase the nutrient levels available to the mycelium immediately after inoculation.
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Holydiver
Registered: 03/19/01
Posts: 4,986
Loc: The midnight sea
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Re: Trypticase Soy Broth - uses? [Re: Holydiver]
#8424542 - 05/20/08 03:40 PM (4 months, 19 days ago) |
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Just revived a 2 year old shaggy mane LC using this stuff, it could be pure coincidence but I highly doubt it. The shaggy mane has been sitting in a vacuutainer at full LC strength, and refrigerated. It looked like dead floating tissue, the kind I usually toss because I have had little luck getting an 8 month old LC to revive let alone 2 years, ESPECIALLY anything that has been sitting around at full strength.
3 days in a 0.01% concentration of soy broth @78F and it is one alive and kicking LC. Pure luck?
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To find a place to live between the negatives and positives.
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MycoAu
Registered: 07/18/07
Posts: 475
Last seen: 10 hours, 12 minutes
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Re: Trypticase Soy Broth - uses? [Re: Holydiver]
#8424638 - 05/20/08 04:08 PM (4 months, 19 days ago) |
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might try plating some to make sure it's not just contaminates of some type. If you were careful, you should be fine. It's not unheard of to recover years-old samples (sometimes even under less-than-ideal conditions).
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