|
Welcome to the Shroomery Message Board! Please  login or  register to post messages and view our members-only content. You'll gain access to additional forums, encrypted messages, file attachments, board customizations, and much more!
|
 b0red5tiff
THE CANDY KING
   
Registered: 09/16/06
Posts: 6,008
Loc: \m/
Last seen: 50 minutes, 13 seconds
|
 THC inhibits breast cancer cell proliferation through JunD
#8377586 - 05/08/08 01:24 PM (1 month, 27 days ago) |
|
|
link
Tetrahydrocannabinol is a potent inhibitor of proliferation in cultured breast cancer cells and exerts its effect through the JunD transcription factor complex, researchers report.
There is increasing evidence that cannabinoids, the active components of marijuana, possess antitumoral properties by inhibiting proliferation and angiogenesis or promoting apoptosis.
A previous study reported by MedWire News showed that cannabidiol, a non-toxic phytocannabinoid, can inhibit breast cancer spread in a rodent model of the disease.
In the present study, Cristina Sanchez (Complutense University, Madrid, Spain) and colleagues turned their attention to the more potent plant-derived cannabinoid, Δ9-tetrahydrocannabinol (THC).
The researchers treated EVSA-T human breast cancer cells with increasing concentrations of THC up to 5 µm for 24 hours and analyzed gene expression profiles using microarray analysis.
THC inhibited the proliferation of EVSA-T cells in a dose-dependent manner.
A panel of 28 genes showed differential expression in response to THC treatment, of which 12 were downregulated and 16 were upregulated with increasing THC concentration.
Three of the upregulated genes were transcription factors, namely Id2, JunD, and Zfp36L1.
Since Id2 had previously been investigated in the study on cannabidiol, the researchers focused on JunD.
Real-time quantitative polymerase chain reaction analysis confirmed that JunD expression was upregulated around 2.6-fold in THC-treated EVSA-T cells relative to non-treated cells. Interestingly, THC had no effect on non-malignant human mammary epithelial cells.
Next, the researchers used confocal microscopy to track radiolabeled JunD protein within EVSA-T cells. They found that JunD was localized to the nucleus upon THC treatment, consistent with its role as a DNA-binding transcription factor.
Finally, the researchers ablated JunD expression in EVSA-T cells using a complementary short interfering RNA signal. THC treatment had little antiproliferative effect on these cells. Notably two genes previously unregulated by THC, namely, cyclin-dependent kinase inhibitor p27 and the tumor suppressor gene testin, were static in cells where JunD was blocked.
"These findings point therefore to a new target to inhibit breast cancer progression, which may contribute to the design of efficient treatments for this malignancy,' Sanchez et al conclude in the journal Oncogene.
--------------------
|
 badchad
Stranger
Registered: 03/02/05
Posts: 2,260
|
Re: THC inhibits breast cancer cell proliferation through JunD [Re: b0red5tiff]
#8377656 - 05/08/08 01:37 PM (1 month, 27 days ago) |
|
|
I always support basic research, but results using cultured cells are a long way from results performed in humans.
|
DrOli
Biochemist
Registered: 08/27/07
Posts: 65
Last seen: 35 minutes, 20 seconds
|
Re: THC inhibits breast cancer cell proliferation through JunD [Re: badchad]
#8377935 - 05/08/08 02:51 PM (1 month, 27 days ago) |
|
|
That still means it is probably closer to more solid evidence for medical cannabis than not doing the research. If you get the cancer vote for the use of cannabis for the cancer growth itself and not symptoms of other medicines then people, (by that I mean biggoted, paranoid, religious politicians), are more likely to take it seriously
|
spock1
Stranger
Registered: 04/14/07
Posts: 589
Last seen: 15 days, 10 hours
|
Re: THC inhibits breast cancer cell proliferation through JunD *DELETED* [Re: DrOli]
#8378192 - 05/08/08 04:07 PM (1 month, 27 days ago) |
|
|
Post deleted by spock1Reason for deletion: .
|
Invisible_Woe
CollectingIntellects AndExhaling SolarSystems
Registered: 05/25/07
Posts: 272
Loc: Standing At The Corner Of...
Last seen: 2 days, 17 hours
|
Re: THC inhibits breast cancer cell proliferation through JunD [Re: spock1]
#8379748 - 05/08/08 10:02 PM (1 month, 27 days ago) |
|
|
i thought this was already known....?
--------------------
The world is like a ride at an amusement park and when you choose to go on it you think it's real, because that's how powerful our minds are. And the ride goes up and down and round and round and it has thrills and chills and it's very brightly colored and it's very loud. And it's fun for a while Some people have been on the ride for a long time and they begin to question: Is this real? Or is this just a ride? And other people have remembered and they come back to us and they say Hey! Don't worry don't be afraid ever because this is just a ride. And we kill those people.Shut him up! We have a lot invested in this ride! Look at my furrows of worry look at my big bank account and my family. This has to be real.
www.zeitgeistmovie.com
| |
|
|
You cannot start new topics / You cannot reply to topics
HTML is disabled / UBBCode is enabled
Moderator: veggie, Carlito
291 topic views. 4 registered and 6 anonymous users are browsing this forum.
[ Toggle Favorite | Print Topic ]
| | |
|
|
Previous
Index
Next
Threaded
Edit 
Reply 
Quote 
