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 camplo
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 Is grain safer than agar?
#8461863 - 05/29/08 10:11 PM (5 months, 30 days ago) |
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Meaning, Say I got a contamed needle right. Its contam is some type of bacteria that doesn't seem to inhibit the young freshly started germinated spores...
Maybe this bacteria would not agree with the challenges of surviving in the ecosystem of the rye jar giving the mushroom mycelial and advantage an thus the mushroom mycelium ultimately over-taking everything. And then I could use the mycelium of the fruits to start a pure culture. yes?
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The shroomy 1
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Registered: 03/27/07
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Re: Is grain safer than agar? [Re: camplo]
#8461905 - 05/29/08 10:20 PM (5 months, 30 days ago) |
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A contaminated syringe is a Failure from the beginning. The "Ecosystem" of the Rye jar is the same Ecosystem for all other competing organisms. Bacteria, mold, virus will thrive in the same environment that mushroom mycelium thrive on. Bacteria multiply exponentially,so trying to "beat" it is pointless.
The Shroomy 1
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camplo
Freedom!
Registered: 02/10/08
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Well I didnt know that 
Spores101 sent me a free contamed syringe? Thats almost funny.
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The shroomy 1
Let me be YOUR"guide"
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Loc: Texas
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Re: Is grain safer than agar? [Re: camplo]
#8469171 - 05/31/08 11:07 PM (5 months, 28 days ago) |
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If I read the subject right, you are asking if grain is safer than agar. That leads me to believe that you have recently had a failed attempt on agar and are questioning if grain would be better because of the seemingly different "ecosystem". First off, working with agar and petri dishes requires a much higher level of sterile procedures. If you don't have a laminar flowhood or a glove box, I wouldn't even attempt working with agar. Second, working with agar is, (in my opinion), exclusive to spore prints, (not spore syringes). I'm not saying that it can't be done or it shouldn't be done, but it compounds the contamination factor. You have to know that with agar even breathing close to the petri dish will probably result in contamination. Hell, I have had un-inoculated dishes contaminate just in the storage phase! I guess what I'm trying to say is that it is so easy to unjustly blame the vendor, when it is most likely our sterile procedure. I exclusively buy from "Ralphsters" and have never bought from Spores 101, but I wouldn't be so quick to pass the blame. Just my thoughts.
The Shroomy 1
--------------------
http://www.sporeworksgallery.com/Cubensae/Psilocybe_cubensis_Texas_White
MY BABY! MINE!
http://www.shroomery.org/forums/showflat.php/Cat/0/Number/9285870/an/0/page/0/gonew/1#UNREAD---Newcomers Guide.
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camplo
Freedom!
Registered: 02/10/08
Posts: 436
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I agree with you, and I would not want to point the figure at anyone who does not deserve. I already feel like I get discredited because I am a noob but it is what it is.
I use a plastic tub style glovebox that has bread bags for arm so when its closed its completely closed off to outside enviroment. I treat the inside of the glovebox with bleachwater and allow 20minutes atleast for the bleach to have time to react also allowing inside air to settle.
My last three inoculations of this style have been successful in the regards that I havent introduced any contams.
I actually did a transfer in this glovebox without the bread bags and it was successfull that I transfered contams from one jar to next but no new ones 
I came to that conclusion because the cobweb looking arial mycelium only stemmed from the tissure transfered and no other spots on the agar sprouted contam...
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RogerRabbit
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Re: Is grain safer than agar? [Re: camplo]
#8476038 - 06/02/08 06:15 PM (5 months, 27 days ago) |
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Grains are for expanding mycelium. Agar is for cleaning up mycelium from contaminants. They're not even remotely similar. Place a drop or two of that syringe on agar, and then transfer healthy mycelium away from the bacteria to a new petri dish. Repeat a few times until you have a clean culture. Then, place that clean culture on grains to grow for spawn.
RR
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