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 spearingflame03
~Gone with thewind~
 
Registered: 03/07/06
Posts: 206
Last seen: 4 months, 5 days
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 Good luck to me right ????
#8378578 - 05/08/08 05:41 PM (6 months, 21 days ago) |
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I prepared 6 1/2pt jars .The pf tek.Pc for about n hour.I will then let the pc cool down naturally while having the jars inside still and still wait for 6+ hours for them to be cool to the touch.Ive been trying to be sterile at the same time with everything.I have lysol and wipes and gloves.
Maybe early 2marrow I will knoc them up.
I have a butane lighter also for flame sterilizing the spore syringe. Any good tips on anything thanks you guys.
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I always have ideas that are conjured up from the manifestations deep within my imagination."Simple but complex".
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scyrusurcys
InformationalNoob
Registered: 03/13/06
Posts: 186
Last seen: 21 hours, 53 minutes
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From opening your PC to setting them in your incubation environment, you must be absolutely sterile with every movement you make.
It doesn't take luck to grow mushrooms, it takes knowledge and practice of the hobby.
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-Scyrus
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 shroober
Myco Junkie
Registered: 01/02/08
Posts: 782
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 Re: Good luck to me right ???? [Re: scyrusurcys]
#8378639 - 05/08/08 05:59 PM (6 months, 21 days ago) |
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Quote:
scyrusurcys said:
From opening your PC to setting them in your incubation environment, you must be absolutely sterile with every movement you make.
unless your entire house is in front of one giant laminar flow hood with a massive UV lamp shining on every inch of surface this is impossible, you need to be clean with your transition from the PC to incubation but once you open the PC it's impossible to remain sterile, that's why you properly prepare the jar lids and then once inoculated wrap coffee filters on the top for extra protection
i think i understand what you are saying but you need to realize that "absolutely sterile" is a very specific term
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Shroober's Lab | Rich Man's Pod | Martha | JumboTub | Outdoor Beds | Flow Hood | Bulk Substrate | Results
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kydub
AmateurMycologist
Registered: 03/12/08
Posts: 104
Last seen: 2 months, 16 days
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Re: Good luck to me right ???? [Re: shroober]
#8378661 - 05/08/08 06:03 PM (6 months, 21 days ago) |
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And nuke the hell out of the air with Oust - or Lysol, if that's all you have. Wearing a filter mask or a bandanna over your mouth/nose while you work helps alot too - your orifices move alot of air.
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Holydiver
Registered: 03/19/01
Posts: 4,997
Loc: The midnight sea
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No you don't have to be sterile in everything you do, thats silly. Besides, you cant come close to a sterile environment at home unless you are working in your PC during a cycle or in front of the flowhood, so the word is misused. Part of the idea of PC'ing stuff in jars is a self contained, sterile environment that remains so until tampered with, or until it just expires and growth eventually takes place. People spraying Lysol all over the place is just absurd and don't seem to understand the micro environment. Most mistakes are made during the transfers from xyz to xyz, where people blame their spore syringe supplier instead of really knowing what's taking place.
Use common sense and good aseptic practices when you are doing clean work. Wipe off the area to be inoculated with alcohol, quickly follow with needle and a good wipe with alcohol soaked cotton swab, etc.
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To find a place to live between the negatives and positives.
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spearingflame03
~Gone with thewind~
Registered: 03/07/06
Posts: 206
Last seen: 4 months, 5 days
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Re: Good luck to me right ???? [Re: scyrusurcys]
#8378701 - 05/08/08 06:11 PM (6 months, 21 days ago) |
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Thanks for the words of incouragement. just took them out and put them straight into my soon to be fc.Jars was warm still so maybe 2marrow will be forsure when i will knoc em up.hehe
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I always have ideas that are conjured up from the manifestations deep within my imagination."Simple but complex".
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kydub
AmateurMycologist
Registered: 03/12/08
Posts: 104
Last seen: 2 months, 16 days
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Re: Good luck to me right ???? [Re: Holydiver]
#8378719 - 05/08/08 06:15 PM (6 months, 21 days ago) |
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Quote:
Holydiver said:
People spraying Lysol all over the place is just absurd and don't seem to understand the micro environment.
Would you mind helping those of us who lack this understanding, then? If lysol/oust is ineffective could you explain why?
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scyrusurcys
InformationalNoob
Registered: 03/13/06
Posts: 186
Last seen: 21 hours, 53 minutes
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Re: Good luck to me right ???? [Re: kydub]
#8378743 - 05/08/08 06:20 PM (6 months, 21 days ago) |
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I just stated to be very sterile so that new growers can learn about the environment they should work in. Even if you don't have the place 100% sterile and contam-free, you've still done more than needs to be done.. and that's how to steer very clear of contaminants.
As for any other tips, as soon as your jars are fully colonized, wait another week... then...
DUNK AND ROLL
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-Scyrus
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spearingflame03
~Gone with thewind~
Registered: 03/07/06
Posts: 206
Last seen: 4 months, 5 days
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Re: Good luck to me right ???? [Re: scyrusurcys]
#8378808 - 05/08/08 06:37 PM (6 months, 21 days ago) |
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thanks man.Looking forward to this.At the same time it does take time.but o'l well 1 step at a time.
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I always have ideas that are conjured up from the manifestations deep within my imagination."Simple but complex".
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mephist00
MuSHRooMeR
Registered: 04/15/08
Posts: 223
Last seen: 1 month, 28 days
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good luck, hope things go well for ya!
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"feed your head!!"
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meatman
hot beef injector
Registered: 04/22/01
Posts: 332
Loc: Underwater Lake
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Re: Good luck to me right ???? [Re: mephist00]
#8379041 - 05/08/08 07:43 PM (6 months, 21 days ago) |
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be patient. do not push your grow, let it do it's thing. good things will happen.
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Everything I post is a complete fabrication made for your entertainment.
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zathan
Denim Slinger
Registered: 08/22/06
Posts: 901
Loc: So long stinktown
Last seen: 1 month, 18 days
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Re: Good luck to me right ???? [Re: kydub]
#8379265 - 05/08/08 08:21 PM (6 months, 20 days ago) |
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Quote:
kydub said:
Quote:
Holydiver said:
People spraying Lysol all over the place is just absurd and don't seem to understand the micro environment.
Would you mind helping those of us who lack this understanding, then? If lysol/oust is ineffective could you explain why?
Because lysol/oust don't do a damn thing to destroy mold spores, which is the most common contaminant. It can prevent mold spores by destroying the mold the creates it. But this would mean you would need to prep your area well in advance, not while you are working on your spawn.
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GrowBuddy
Grow like a pro.
Registered: 02/29/08
Posts: 109
Last seen: 3 months, 26 days
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Re: Good luck to me right ???? [Re: meatman]
#8379266 - 05/08/08 08:21 PM (6 months, 20 days ago) |
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Good luck, stay clean! 
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The best how-to grow guide with the first ever mushroom cultivating software.
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shroober
Myco Junkie
Registered: 01/02/08
Posts: 782
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Re: Good luck to me right ???? [Re: GrowBuddy]
#8379548 - 05/08/08 09:15 PM (6 months, 20 days ago) |
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Quote:
GrowBuddy said:
Good luck, stay clean!
I'm starting to think you dont even read the posts here, all your posts say something along the lines of "Good Luck", "Nice Job", "Looking Good". I'm starting to think you're just shamelessly promoting your little software. If that is your intention then just become a vendor, stop adding these annoying, meaningless posts.
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Shroober's Lab | Rich Man's Pod | Martha | JumboTub | Outdoor Beds | Flow Hood | Bulk Substrate | Results
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12468
Stranger
Registered: 12/04/07
Posts: 287
Last seen: 18 hours, 30 minutes
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Re: Good luck to me right ???? [Re: shroober]
#8379565 - 05/08/08 09:18 PM (6 months, 20 days ago) |
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I don't know, my friend's sterile procedure is a joke and my friend's house is filthy, he's never had a problem with contams at all.
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kydub
AmateurMycologist
Registered: 03/12/08
Posts: 104
Last seen: 2 months, 16 days
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Re: Good luck to me right ???? [Re: zathan]
#8379863 - 05/08/08 10:26 PM (6 months, 20 days ago) |
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Quote:
Holydiver said:
Because lysol/oust don't do a damn thing to destroy mold spores, which is the most common contaminant. It can prevent mold spores by destroying the mold the creates it. But this would mean you would need to prep your area well in advance, not while you are working on your spawn.
Understood - I generally 'nuke' the area about 30-40 minutes before I start, and then again once I've placed all my materials (PC, torch, ect ect) in my working area and spray again, and let sit for another 10 or so before sitting down to begin. Still ineffective?
When you say 'destroying the mold the creates it' are you talking about sterilizing the house itself, the actual sources of mold - or just what might be in/around the working area?
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spearingflame03
~Gone with thewind~
Registered: 03/07/06
Posts: 206
Last seen: 4 months, 5 days
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Re: Good luck to me right ???? [Re: kydub]
#8379896 - 05/08/08 10:34 PM (6 months, 20 days ago) |
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thats a good question because i would like to know also. my jars are cooling down in my soon to be fc.to late for me to inject them.so ill wait till tomarrow..Ill hit the whole room with lysol 30 min b4 i do the inection then do it again b4 i really do inject. I like that metheod hope you dnt mind if i borrow this.
I have just a lil heater n my room i use when its a lil chilly could i use this in any ways? I was thinking of once i get down injecting the spores to cover the jars in garbage bags and rap a towel around them.and have my heater hiting the towel making it warm ? inputs ? thanks ??
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I always have ideas that are conjured up from the manifestations deep within my imagination."Simple but complex".
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Jeeezelouise
Mazer
Registered: 04/19/08
Posts: 248
Last seen: 4 months, 29 days
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Re: Good luck to me right ???? [Re: Holydiver]
#8380320 - 05/09/08 12:18 AM (6 months, 20 days ago) |
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Quote:
Holydiver said:
No you don't have to be sterile in everything you do, thats silly. Besides, you cant come close to a sterile environment at home unless you are working in your PC during a cycle or in front of the flowhood, so the word is misused. Part of the idea of PC'ing stuff in jars is a self contained, sterile environment that remains so until tampered with, or until it just expires and growth eventually takes place. People spraying Lysol all over the place is just absurd and don't seem to understand the micro environment. Most mistakes are made during the transfers from xyz to xyz, where people blame their spore syringe supplier instead of really knowing what's taking place.
Use common sense and good aseptic practices when you are doing clean work. Wipe off the area to be inoculated with alcohol, quickly follow with needle and a good wipe with alcohol soaked cotton swab, etc.
Having many years experience canning foods and brewing mead, I see a LOT of redundant sterilization steps in most of the teks, you're right. But I'm not going to say what those redundant steps are, because everyone should learn enough about the whole business of encouraging fungi and discouraging bacteria that they can see for themselves what is redundant 
regards
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bigcraig77
dutch's 4 life
Registered: 11/09/07
Posts: 96
Loc: central mass
Last seen: 25 days, 4 hours
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Re: Good luck to me right ???? [Re: Jeeezelouise]
#8380983 - 05/09/08 07:52 AM (6 months, 20 days ago) |
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I noc my jars next to my cats litter pan.
i spray the air down with lysol and never have any contams.
who knows. maybe im lucky.
oh but i do use a make shift glove box.
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they must find it difficult .... those who have taken authority as the truth, rather than truth as the authority" - Gerald Massey
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zanny787
_*_
Registered: 07/09/07
Posts: 21
Loc: buffalo/colorado
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Re: Good luck to me right ???? [Re: Jeeezelouise]
#8381034 - 05/09/08 08:33 AM (6 months, 20 days ago) |
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Quote:
Jeeezelouise said:
Quote:
Holydiver said:
No you don't have to be sterile in everything you do, thats silly. Besides, you cant come close to a sterile environment at home unless you are working in your PC during a cycle or in front of the flowhood, so the word is misused. Part of the idea of PC'ing stuff in jars is a self contained, sterile environment that remains so until tampered with, or until it just expires and growth eventually takes place. People spraying Lysol all over the place is just absurd and don't seem to understand the micro environment. Most mistakes are made during the transfers from xyz to xyz, where people blame their spore syringe supplier instead of really knowing what's taking place.
Use common sense and good aseptic practices when you are doing clean work. Wipe off the area to be inoculated with alcohol, quickly follow with needle and a good wipe with alcohol soaked cotton swab, etc.
Having many years experience canning foods and brewing mead, I see a LOT of redundant sterilization steps in most of the teks, you're right. But I'm not going to say what those redundant steps are, because everyone should learn enough about the whole business of encouraging fungi and discouraging bacteria that they can see for themselves what is redundant
regards
hahah thats so stupid.
Like saying "i know something you dont know" and running off giggling.
Riight.
Why did u even bother posting then??
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