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 Gobblin
Crazy Fingers
Registered: 05/13/05
Posts: 1,592
Loc: South Saturn Delta
Last seen: 19 hours, 28 minutes
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 Ports and grass seed; another failure?
#8364030 - 05/05/08 04:49 AM (6 months, 13 days ago) |
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What the hell am I doing wrong????
Day 7 of 6 pint jars of grass seed soaked for 24 hours and PC'd @ 15psi for 90 min.
Knocked up with an LC of Torks, incubating at 82 degrees. NO signs of LIFE!!!!
This is the second failure for this strain. Last one was with WBS.
Suggestions please...
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Reality is a figment of your hallucination
  
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Hotnuts
old hand
Registered: 02/26/05
Posts: 2,748
Loc: Wild Blue Yawnder
Last seen: 12 hours, 55 minutes
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 Re: Ports and grass seed; another failure? [Re: Gobblin]
#8364284 - 05/05/08 08:34 AM (6 months, 13 days ago) |
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82 is too hot. Most of the species of agaricus that are cultivated prefer temperatures in the 72-74 range for incubation. I'd think those would as well. Is that a Sporeworks strain?
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Gobblin
Crazy Fingers
Registered: 05/13/05
Posts: 1,592
Loc: South Saturn Delta
Last seen: 19 hours, 28 minutes
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Re: Ports and grass seed; another failure? [Re: Hotnuts]
#8364953 - 05/05/08 12:55 PM (6 months, 13 days ago) |
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Yes, and it's supposed to be a warm weather strain, hence the warm coloniztion temps.
check it out
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Reality is a figment of your hallucination
Edited by Gobblin (05/05/08 12:57 PM)
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Hotnuts
old hand
Registered: 02/26/05
Posts: 2,748
Loc: Wild Blue Yawnder
Last seen: 12 hours, 55 minutes
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Re: Ports and grass seed; another failure? [Re: Gobblin]
#8365070 - 05/05/08 01:31 PM (6 months, 13 days ago) |
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Oh, I didn't realize they had that.
Maybe you should shoot Workman a pm so he catches this thread and can give you some advice on it.
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 2,387
Loc: Washington, USA
Last seen: 2 hours, 13 minutes
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Re: Ports and grass seed; another failure? [Re: Hotnuts]
#8365088 - 05/05/08 01:42 PM (6 months, 13 days ago) |
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If its a spore works culture, I'd suggest asking for a replacement. It is a slow starter so it may be just lagging but it sounds more like a bad syringe. Don't give up hope yet.
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Research funded by the patrons of
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Reinvesting 25% of Sales Towards Basic Research and Species Identification 
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Gobblin
Crazy Fingers
Registered: 05/13/05
Posts: 1,592
Loc: South Saturn Delta
Last seen: 19 hours, 28 minutes
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Re: Ports and grass seed; another failure? [Re: Workman]
#8365222 - 05/05/08 02:26 PM (6 months, 13 days ago) |
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PM sent.
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Reality is a figment of your hallucination
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makaveli8x8
Stranger
Registered: 02/28/06
Posts: 6,739
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Re: Ports and grass seed; another failure? [Re: Gobblin]
#8366133 - 05/05/08 07:10 PM (6 months, 13 days ago) |
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i had to leave my jars sit for 1-2 months after shooting them up to finally see growth, after I finally seen growth they took off vary similar to others, might have been some bacteria contamination in there
anyways if you wait i bet you will see growth eventually then just transfer to agar or w/e
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  We were sent to hell for eternity  Ø h®
We play on earth to pass the time 
Over-population the root of all Evil-brings the Elites Closer to the gates.
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Gobblin
Crazy Fingers
Registered: 05/13/05
Posts: 1,592
Loc: South Saturn Delta
Last seen: 19 hours, 28 minutes
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Re: Ports and grass seed; another failure? [Re: makaveli8x8]
#8366188 - 05/05/08 07:26 PM (6 months, 13 days ago) |
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1-2 months just to see any growth  ...thats pretty freaking slow...
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Reality is a figment of your hallucination
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makaveli8x8
Stranger
Registered: 02/28/06
Posts: 6,739
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Re: Ports and grass seed; another failure? [Re: Gobblin]
#8366258 - 05/05/08 07:41 PM (6 months, 13 days ago) |
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yah well like i said it pretty much grew like any other after that point, prob contam holding it back 
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We were sent to hell for eternity Øh®
We play on earth to pass the time
Over-population the root of all Evil-brings the Elites Closer to the gates.
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 2,387
Loc: Washington, USA
Last seen: 2 hours, 13 minutes
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Re: Ports and grass seed; another failure? [Re: makaveli8x8]
#8368732 - 05/06/08 12:47 PM (6 months, 12 days ago) |
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I have experienced the same thing. Its leapoff lag. Agaricus seems to have a hard time when the substrate is changed. We may need to alter the liquid media to match the most commonly used substrates.
--------------------
Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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