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 dstark
Yes you can but you may Not!
Registered: 02/27/08
Posts: 1,125
Loc: -CamP!ng iN mY Ro.Om-
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 Portabello spore germination!
#8309712 - 04/21/08 05:52 AM (7 months, 7 days ago) |
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So i have 3 jars with substrat of: Rice flour, Saw dust and Vermiculite. Passed 5 days and there is so sign for myc!
Im worried :P Maybe it to early to talk but i insolated at the same day PC burma strain and there in myc grow!
dstark.
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.:Darkie:.
Sucks my Sig must be only 65kb ;(
Edited by dstark (04/21/08 06:02 AM)
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 2,399
Loc: Washington, USA
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 Re: Portabello spore germination! [Re: dstark]
#8310100 - 04/21/08 10:32 AM (7 months, 7 days ago) |
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Agaricus spores don't readily germinate in sterile substrates. Assuming you used actual spores and not a liquid culture, you may never see any growth. There are tricks to get Agaricus spores going on agar such as actived carbon and certain bacteria. The best results I have had was by placing growing mycelium on one side of a the petri dish and spores on the other. The actively growing mycelium stimulates the spore germination.
If you actually meant a liquid culture instead of spores, you should see growth in a couple of weeks. They are much slower than cubensis.
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dstark
Yes you can but you may Not!
Registered: 02/27/08
Posts: 1,125
Loc: -CamP!ng iN mY Ro.Om-
Last seen: 23 hours, 39 minutes
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Re: Portabello spore germination! [Re: Workman]
#8310167 - 04/21/08 10:59 AM (7 months, 7 days ago) |
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Thanks for reply 
Nope it was spores! i also puted some on petri dish and there is grow only after 2 weeks :O
Bah so shall i make liquide culture??? O.o
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.:Darkie:.
Sucks my Sig must be only 65kb ;(
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Workman
Psilocybe Microscopist
Registered: 03/01/01
Posts: 2,399
Loc: Washington, USA
Last seen: 7 hours, 31 minutes
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Re: Portabello spore germination! [Re: dstark]
#8310604 - 04/21/08 01:31 PM (7 months, 7 days ago) |
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Putting massive amounts of spores on agar can work sometimes. It just takes one spore to germinate to set off the rest. But it might also be contamination so watch it close. Maybe you will get lucky in your jars as well.
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Research funded by the patrons of
The Spore Works
Exotic Spore Supply
Reinvesting 25% of Sales Towards Basic Research and Species Identification
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Cryogenicz
Vendor
Registered: 07/01/04
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Loc: Oregon
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Re: Portabello spore germination! [Re: Workman]
#8315628 - 04/22/08 06:41 PM (7 months, 6 days ago) |
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I would run that mycelium out on agar, and then transfer it to grain.
-Graham
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dstark
Yes you can but you may Not!
Registered: 02/27/08
Posts: 1,125
Loc: -CamP!ng iN mY Ro.Om-
Last seen: 23 hours, 39 minutes
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Re: Portabello spore germination! [Re: Cryogenicz]
#8325919 - 04/25/08 02:48 AM (7 months, 3 days ago) |
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Okayz so today i moving some portabello myc from 1 petri dish to another! reason is that there is contam in the petri...
Wish me luck :P
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.:Darkie:.
Sucks my Sig must be only 65kb ;(
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