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 SuchSmartMonkeys
mycologicallydrivenindividual
Registered: 10/26/05
Posts: 535
Last seen: 4 days, 4 hours
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 coffee substrate for oysters.....Qs?
#8343984 - 04/29/08 09:49 PM (6 months, 30 days ago) |
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okay, so i'm planning on making a shit ton of bags of coffee substrate to for oysters... I'm going to go to starbucks and ask for a garbage bag full of grounds, and fill as many bags as i can, and autoclave them, then transfer grain spawn to said bags, colonize, and fruit. Here are my questions:
anybody have any experience with pure coffee substrate for oysters? will i need to supplement the coffee with anything (gypsum or lime? or something that will give the substrate more body? i have a real old bag of straw that's been sitting outside....) or will the straight coffee grounds be fine as a substrate medium? and i think last but not least, how long will i need to autoclave these bags for, will 50 minutes in the autoclave do, or am i going to have to run a 50 minute and a 30 minute cycle back to back? (pain in the ass, this is the only settings it has for now.... i'm gonna figure out how to change the settings next week.)
I have 20 fully colonized jars, none of them contaminated, i'm going for the largest scale grow that i've ever done... going to be giving away a lot of fully spawned, ready to fruit kits to friends and family members, maybe i'll give a few away on here, or make some trades...
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 18,204
Loc: USA Mountain Northwest
Last seen: 27 minutes, 7 seconds
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I'd suggest mixing the coffee with straw. Add up to ten percent gypsum by volume, and DON'T autoclave. Pasteurize at 140F for an hour to 90 minutes.
RR
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SuchSmartMonkeys
mycologicallydrivenindividual
Registered: 10/26/05
Posts: 535
Last seen: 4 days, 4 hours
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Re: coffee substrate for oysters.....Qs? [Re: RogerRabbit]
#8344198 - 04/29/08 10:24 PM (6 months, 30 days ago) |
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alright, i haven't that much straw, and what i have is pretty old, some of it's got a bit of mold on it..... would it be possible to do just coffee bags, or am i really going to need some more structure in there?
and the amount of bags i'm planning on doing, i have no way of easily pasteurizing, it would be scads easier to just toss them all into the big clave i have access to, it's pretty big. I'll think about this one.... if i were to do so, what would be the downside, i know the upside being more beneficial bacteria at the fruiting stage...?
I'll mix in 10% gypsum, but also, how should i go about hydrating this stuff?
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RogerRabbit
Bans for Pleasure
Registered: 03/26/03
Posts: 18,204
Loc: USA Mountain Northwest
Last seen: 27 minutes, 7 seconds
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Hydrate to field capacity. If you don't use straw, I'd suggest at least to mix the coffee grinds with up to 50% vermiculite or sawdust. Don't use moldy straw.
As I said earlier, pasteurize, don't sterilize bulk substrates or you run a much higher risk of contamination.
RR
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www.mushroomvideos.com
"I wouldn't want to belong to any club that would have me as a member".
Mark Twain, Woody Allen, Groucho Marx, and anyone else who wishes to claim credit for the quote.
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SuchSmartMonkeys
mycologicallydrivenindividual
Registered: 10/26/05
Posts: 535
Last seen: 4 days, 4 hours
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Re: coffee substrate for oysters.....Qs? [Re: RogerRabbit]
#8345650 - 04/30/08 10:43 AM (6 months, 29 days ago) |
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i don't understand exactly.... could you clarify why exactly there is higher risk of contam if the fruiting/bulk sub is sterilized instead of pasteurized? i always read that pasteurizing was good because it inactivates all the bacteria and such, which becomes active again at the fruiting stage (if you've timed it right) and it's beneficial for flushes... Sterilization just kills everything. could you please explain? thanks Roger!
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x7x_x7x
urban farmer
Registered: 05/05/07
Posts: 681
Last seen: 1 day, 15 hours
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how much you pay for the coffee?
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transhumanism + primitivism=magic mushrooms
SuctoSporeŽ
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Andrew47
Servant of allLife
Registered: 04/06/06
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Re: coffee substrate for oysters.....Qs? [Re: x7x_x7x]
#8400183 - 05/14/08 10:01 AM (6 months, 15 days ago) |
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Pasteurization allows high temperature microbes to gain hold of the substrate and prevent foreign spores from germinating. These organisms protect the substrate to a point from contamination. Colonized spawn is so strong, though, that it quickly takes hold and is excited by competition with other microbes
If you pasteurize a bulk substrate you can spawn to it in any reasonably clean environment, including outside. If you sterilize your bulk substrate you have to use the same procedures you would for a grain to grain transfer, requiring a glovebox or preferably a flow hood.
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SuchSmartMonkeys
mycologicallydrivenindividual
Registered: 10/26/05
Posts: 535
Last seen: 4 days, 4 hours
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Re: coffee substrate for oysters.....Qs? [Re: Andrew47]
#8407395 - 05/15/08 10:12 PM (6 months, 14 days ago) |
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oh yeah, no big deal, i did it all in a flowhood, and i don't see any contamination in any of my 15 bags..... they're almost fully colonized.
and to x7x, i didn't pay shit for the coffee, i just went to starbucks and asked them for their spent coffee grounds. it's pretty sweet, their machines are fully automatic, and every time they make an espresso, it just kicks a little puck of spent grounds into this holding receptacle. they just dump out shit tons of them... they were happy to give me a garbage bag full....
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x7x_x7x
urban farmer
Registered: 05/05/07
Posts: 681
Last seen: 1 day, 15 hours
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ok! i got three blocks of sawdust colonized with oysters, with disapointing results, the first block give only one (but beatiful) handful of fruits- util now. lets see what happen the next week. they need coffee, straw or some shit like that.
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transhumanism + primitivism=magic mushrooms
SuctoSporeŽ
Edited by x7x_x7x (05/16/08 10:39 AM)
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x7x_x7x
urban farmer
Registered: 05/05/07
Posts: 681
Last seen: 1 day, 15 hours
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Re: coffee substrate for oysters.....Qs? [Re: RogerRabbit]
#8408908 - 05/16/08 10:43 AM (6 months, 13 days ago) |
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Quote:
RogerRabbit said:
If you don't use straw, I'd suggest at least to mix the coffee grinds with up to 50% vermiculite or sawdust.
As I said earlier, pasteurize, don't sterilize bulk substrates or you run a much higher risk of contamination.
RR
sawdust could be pasteuized or need sterilization?
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transhumanism + primitivism=magic mushrooms
SuctoSporeŽ
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