What is the longest I should wait for mycilium to colonize before I get rid of the jar

Is this tek outdated?

What should I use on the floor of the fruiting chamber for the ultrasonic humidifier setup? I was thinking perlite but if i use that then what use would the humidifier be, right? whats a good non absorbent cushioning? Should i just use  vermiculite? Thanks for this tutorial buy the way it has helped so much.

I followed this guide exactly, the cake mixture part at least, and my cakes did great. They colonized with B strain in about a month and because of sterility tips I took from other guides, there were zero contaminated jars. The cakes shrunk a little bit as they colonized and slid out very smoothly. I used a poor man's terranium in this guide which the first flush provided 70 grams even, wet of course. I used perlite at the bottom of the 2 liter to keep it very humid and also cut holes at the top and bottom just above the perlite layer so I don't have to fan it much. My other terranium can support more cakes, but I only have four at a time and they are still going. It's slower than the 2 liter but there are many more pins on each cake because of the extra room. None have been picked yet, but every time I look at it I see progress. My first ever mushroom trip will be this sunday with my best friend and I can't wait! This is a great guide, I'll definitely send my regards in green bills when I get a chance.

Actually, 4*50% is 2.

I have a question. During the colonization period you point out that as the fungi grows bigger, its growth rate begins to deteriorate because as they grow they use up more oxygen. Couldn't this problem be easily fixed by punching in additional holes? The additional holes should provide more ventilation the bigger fungi needs, thus allowing it to retain its growth rate, thereby making the entire growth time lower, and everyone likes growing good stuff in less time right?

wow this is awesome :)

"In order to illustrate the point, assume that the spore syringe has slightly 'dirty' contents but is still viable. Assume any given injection site has a 50% chance of becoming contaminated. If you inject only one site, the culture has a 50% chance of becoming contaminated. If you inject four sites, the culture has a .5 * .5 * .5 * .5 = 6%"