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Club99's Honeywater Mycelium Tek

The achievement of this tech is to shorten the time needed to colonize a jar as you'll see signs of growth in 24/36 hours, instead waiting 3/5 days for spore germination.



The achievement of this tek is to shorten the time needed to colonize a jar as you'll see signs of growth in 24/36 hours, instead waiting 3/5 days for spore germination.

What you need:

  • A narrow mouth jar with lid
  • Organic honey - best is the pale yellow one
  • Mineral water
  • Some H2O2 (at 3% or 12 vol.)

Optional:

  • Some heat resistant silicone
  • A filter disk

How to do it:

  1. Drill a small hole in the lid, large enough to pass through with a needle.
  2. Mix a small quantity of honey (less than a teaspoon) in the water, the water must be very light yellow coloured.
  3. Sterilize for 30 minutes and let it cool (don't over sterilize or the sugars in the honey caramelize).
  4. Inject a small quantity of spore solution, 1-2 cc it's enough.
  5. Put in a dark warm place, better to stir a bit once a day, and wait until you see small white hairs floating in the solution,
  6. wait a day or 2 then add some H2O2 , few cc's, (i would put 2 cc in a 1/2 pint jar) then put the jar in the fridge. This helps to keep out contaminants. Specially bacteria.
  7. Now the honeywater it's ready. To use it simply suck some water with a sterile syringe and inoculate your MMGG/PF TEK style jars as usual.
  8. The solution will last at least 2 weeks, but anyway i wouldn't use a solution much older as the risks of contamination increases.

Some optional improvements:

  • A drop of silicone closing the hole helps to keep out contaminants.
  • advice!! If you close air tight your jars the sterilization will produce a negative pressure that will suck ALL your syringe into the jar. Best to tight the lids after sterilization.
  • You can drill 2 holes in the lid and use one to inject spores/suck liquid mycelium and the other one for gas exchange as shown in the the pic. (Thanks Dr. Dung for this tip)
Amazon Shop for: ½ Pint Jars

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