Shroomery - Magic Mushrooms Demystified
Scientists have been interested in this drug group for several reasons. Because they produce profound changes in perception and affect, hallucinogenic drugs might provide some insight into these basic psychological processes. Similarly, some consider them to be of particular interest form a mental health perspective since they are psychotomimetic - that is, their effects mimic certain aspects of the major psychoses. Finally, because these impressive effects are often produced by minute quantities (microgram amounts in the case of LSD), hallucinogenic drugs are of particular interest to those who study the brain; their potency implies that the drugs act with specificity at particular sites within the brain.
Although a large number of drugs may be considered psychoactive (i.e., influencing psychological processes such as perception, emotion, memory and attention), only a small group are generally identified as hallucinogenic: LSD (lysergic acid diethylamide), 2,5-dimethoxy-4-methylamphetamine (DOM), N,N-dimethyltryptamine (DMT), Psilocin, mescaline, and their congeners.
Not only is evidence of cross-tolerance important for grouping drugs, but it provides presumptive evidence for a common biological site of action and encourages the strategy of searching for a single site in the brain shared by all of the drugs in the class. From a strictly molecular point of view, it is not obvious that this should be the case, since the various drugs are structurally dissimilar (fig. 2): LSD is an ergot derivative; mescaline and DOM are phenylethylamines; and Psilocin and DMT are indoleamines. However, as we shall see, evidence from basic biological studies does indeed support a common site of action for all of them.
Specification of the location of the cell bodies, axon pathways, and terminals of each of these groups of neurons permitted maps based on neurochemical identify to be drawn. No only did this facilitate the development of more meaningful "wiring diagrams" of the brain, but it attorded scientists the opportunity to study and manipulate particular groups of neurons. The neurons that we shall be examining in detail, those containing serotonin (5-hydroxytryptamine, or 5-HT), have their cell bodies (the point where an action potential is initiated) in a primitive part of the brain called the brainstem (Fig. 4)), which is known to control many basic physiological processes such as respiration and functioning of the cardiovascular system. From this site of origin, the axons of serotonergic neurons project to virtually all portions of the brain, including the most recently evolved neocortex.
One complication of this simple picture of chemical neurotransmission that is of special relevance to our story is the fact that each neurotransmitter can act at more than one type of receptor. It is assumed that these receptor subtypes exist for the purpose of diversifying the cellular effects of any given neurotransmitter. Thus, serotonin acts as 5-HT1A, 5HT1B, possibly 5-HT1C, and 5-HT2 receptor subtypes in the brain (Fig. 5). Because the protein molecules, which constitute receptor sites, have slightly different conformations for each subtype, drugs can be developed to stimulate (or block) a particular receptor subtype. This preferential action presumably does not occur under normal physiological conditions, since the endogenous neurotransmitter is active at all the receptor subtypes.
Hallucinogens and Serotonin
Much of the research on hallucinogenic drugs has focussed on brain serotonin. There are two major reasons for this. First it was discovered early on that many of the major hallucinogens had a molecular structure similar to that of serotonin. Second, animal studies examining brain neurochemistry following administration of hallucinogens invariably reported changes in serotonin. It is not surprising that much of the brain and a number of its neurotransmitters react to the administration of these powerful drugs. However, the only reliable and consistent change common to all LSD-like hallucinogens is seen in brain serotonin, manifesting itself as changes in synthesis, release, catabolism, or receptor action (7).
It is well known that he hallucinatory experience is a varied and complex one - in fact, this is one of the hallmarks of hallucinations. Therefore, it may appear naive to speak of these effects being mediated by a single specific neurotransmitter system. However, we are talking about the primary (or initiating) site of action of hallucinogenic drugs. Once a drug acts upon the brain and many of its constituent neurochemical systems. Thus the brain serotonin system acts as a trigger for a multitude of changes whose elaboration generates the hallucinatory experience
Research on the role of serotonin in the action of hallucinogenic drugs has gone through three distinct phases, The first phase was initiated in the mid-1950s. It was based on the then recently discovered fact that the LSD molecule was structurally similar to that of serotonin. Investigators correctly reasoned that this might imply that l.SD's effects were mediated by an action on the neurotransmission of serotonin in the brain. Unfortunately, the level of technical expertise in the field of brain research was so primitive at that time that this hypothesis had to be tested on peripheral tissue (i.e., outside the brain), Two different groups of scientist reported that LSD exerted a powerful blockade of serotonin's biological action (8, 9). This hypothesis was quickly challenged, however, by studies employing brom-LSD a close structural analogue of LSD with a single bromine atom attached to it (10). Brom-LSD was less potent as LSD in blocking the action of serotonin in the periphery, but it did not cause hallucinogenic activity in humans We now know that the action of a drug at one site in the body does not necessarily generalize to the drug's action at another site, especially when one site is in the brain and the other is not.
In the second phase, the action of hallucinogenic drugs on the brain was examined directly. This work was begun by Daniel Freed man at Yale University in the 1960s. In his neurochemical studies he reported that the administration of hallucinogenic drugs to animals increased the level of brain serotonin and decreased the level of serotonin's metabolic by-products (11). This pattern of results implied that neurons utilizing serotonin for neurotransmission were being turned off or inhibited by the drugs.
Technical advances in the mid-1960s permitted the direct testing of this hypothesis. As mentioned above, a group of Swedish investigators had mapped the location of the cell bodies, axons and terminals of several neurotransmitter systems in the brain, including serotonin. With this information in hand, the next logical step was to insert a recording microelectrode into the brain- stem areas where serotonin neurons are most densely aggregated and to administer a hallucinogenic drug. When George Aghajanian at Yale University did this in anaesthetized rats, he found that the characteristically slow (1 or 2 action potentials/second) and highly regular pattern of electrical activity of these neurons was dramatically suppressed by injections of LSD(12). This supported the theory that I so and related hallucinogens acted by directly suppressing the activity of serotonin neurons themselves-the so-called presynaptic hypothesis.
Although the hypothesis is attractively parsimonious, there are a number of experimental findings at variance with it. For example, our studies of serotonin neurons in awake, freely moving cats have found that the behavioural effects of LSD (e.g., limb flicking, grooming, head shakes, investigatory and play behaviour, and constant eye movements) greatly outlast the suppression of neuronal activity produced by the drug. Furthermore, animals given repeated doses of LSD ultimately develop tolerance, displaying little or no behavioural response, yet the magnitude of the concomitant suppression of serotonin neuronal activity is undiminished (13). Finally, and perhaps most importantly, some of the major hallucinogens, such as mescaline and DOM, produce robust behavioural effects in spite of failure to suppress significantly serotonin neuronal activity (14).
Behavioural pharmacology studies by James Appel at the University of South Carolina also question the presynaptic hypothesis. If the hallucinogenic drugs act by suppressing the activity of brain serotonin neurons, then prior destruction of these neurons (or depletion of serotonin from its axon terminal storage sites) should make the drugs behaviourally inactive, because the system is already maximally suppressed. To the contrary, such manipulations if anything enhance, rather than diminish, the behavioural effects of LSD and related hallucinogens in animals (15-17). Similar results have also been reported in studies on humans (18, 19). These enhanced effects in animals and humans are probably attributable to a proliferation of serotonin receptor sites on the target neurons to which serotonin neurons project. This phenomenon, which often follows neuronal destruction or neurotransmitter depletion, is called "denervation supersensitivity," and the increase in the number of receptor sites appears to be a compensatory response to the decreased input. In this way, synaptic homeostasis is maintained.
Postsynaptic action of hallucinogens
If hallucinogenic drugs do not exert their important action directly on serotonin neurons, is there an alternative hypothesis which still preserves the concept of a serotonin-like effect? The answer is yes, and the data supporting it represent the third (and current) phase of research on serotonin and hallucinogenic drug action. The hypothesis proposes that LSD and related drugs act directly at receptor sites on serotonin target neurons; Serotonin is known to exert both excitatory and inhibitory actions at these sites. One of the most compelling aspects of the data which support this postsynaptic hypothesis is their convergent nature and the number of different laboratories which have contributed to them.
Studies employing radioactively labeled compounds that bind to particular receptors with great specificity and high affinity also point to the importance of postsynaptic serotonin receptors in hallucinogenic drug action. With repeated administration, we found that LSD became less and less effective in eliciting the behavioral syndrome in rats (20. 22). This change was accompanied by a significant decrease in the number of serotonin binding sites available on postsynaptic neurons. The specificity of the effect is demonstrated by the fact that no change was found in the availability of receptors for dopamine, another brain neurotransmitter. Recently, these results have been confirmed and extended in an important way. Repeated administration of LSD to rats was found to decrease the availability of the 5-HT: receptor subtype. Binding at the 5-HT, subtypes was unchanged (23).
Additional evidence favoring the postsynaptic hy-(Hypothesis comes from studies employing monoamine oxidase inhibitors (maois), which are widely used to treat depression in humans. One of the changes that prolonged administration of these drugs produces is a decrease in the number of serotonin receptor binding sites in the brain (24). Irwin Lucki and Alan Frazer at the University of Pennsylvania found that when rats were treated for a number of days with maois and then given drugs, independent of their structure, exert their critical action at a 5-HT2 receptor site (36). They took a series of 22 drugs whose potency to elicit hallucinations in humans was known. The drugs were drawn from the three different structural groups of hallucinogenic drugs: ergot, phenyl ethylamine, and indoleamines. Employing radioactively labelled compounds =, they examined the affinity of these drugs for binding to 5-HT2 receptor sites in brain tissue taken from rat cerebral cortex. They found that the correlation coefficient of human hallucinogenic potency and affinity for binding was nearly perfect. This strongly supports the hypothesis that the strength of a drug's action at the 5-HT2 receptor site predicts its potency in evoking hallucinations in humans.
A critical experiment remains to be carried out as the ultimate test of the hypothesis. Will pre-treatment of human subjects with drugs that are highly specific antagonists of serotonin's action at the 5-HT2 site block, or markedly attenuate, the hallucinogenic effects of LSD, mescaline, DOM, Psilocin, and DMT? We may have to wait a long time for an answer to this question because of federal restrictions on such experiments.
Serotonin and the Brain
Data from a variety of different sources lead to the conclusion that hallucinogenic drugs exert their critical action at a specific serotonin receptor subtype, 5-HT2. (Depending on the particular brain area, the action may be either excitatory or inhibitory.) This does not preclude the possible involvement of other neurotransmitters in the action of hallucinogenic drugs. In fact, structural differences in the drug molecules are probably responsible for variations in the phenomenological! Effects produced by them,
A major remaining issue is how this triggering action of hallucinogenic drugs at 5-HT; receptors evokes the experience we identify as a hallucination-that is, changes the sensory-perceptual, psychic, and somatic domains. As further progress is made in elucidating the neural substrates of emotion, perception, and other processes, we will better understand how a change in the modulatory influence exerted by serotonin at its receptor sites throughout the central nervous system mediates hallucinogenesis. Recent anatomical studies indicate that tilt 3-HT; receptors are abundantly localized in neocortical, limbic, and brainstem sites. In addition, noninvasive scanning techniques for studying brain function in conscious humans, such .is positron emission tomography (PET) and nuclear magnetic resonance (NMR), are proving invaluable to research on psychoactive drugs.
Finally, we may ask what functions are subserved by the other receptor sites where serotonin acts. Recent evidence indicates that one of these sites (5-HT1A) may be important in producing anxiety, while another may be critical in the development of migraines. It is important to remember that under normal conditions serotonin exerts an equal action at all of its receptor sites. However, when one site is selectively activated or blocked, whether by drugs, endogenous biological factors, or environmental toxins, it leads to a perturbation which may be manifested as anxiety, migraines, or hallucinations.
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