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Where should the inoculation take place?

You can inoculate your jars in the "open air" (without any sterilization precautions), using the "oven tek", in a glovebox or under a flow hood.



You can inoculate your jars in many places - in the "open air" (without any sterilization precautions), using the "oven tek", in a glovebox or in front of a flow hood.
Since PF jars are sealed, and there is little chance of introducing a contaminant to the substrate through the 4 holes punched in the lid, all of these methods of inoculation are acceptable.

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